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Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!

Intact mAb Mass Check Standard

Fully Characterized Monoclonal Antibody Standard

Analysis of intact proteins by MS is a powerful approach for protein identification and assignment of protein modifications. The Intact mAb Mass Check Standard is a LC-MS standard used as a qualitative tool for intact mass analysis. This standard is designed to be used for routine MS optimization, system setup and performance testing.

Intact mouse IgG1 protein purified by Protein-A with a known molecular weight
Ideal for higher molecular weight intact mass measurements
Used as the unlabeled, control standard for the GlycoWorks RapiFluor-MS N-Glycan sample preparation workflow as it has 4 major glycosylated peptides
Provided in a Waters TruView Vial (ideal for peptides) as 1 mg of lyophilized solid (lyophilization grade sucrose is used for stabilization).
Recommended solubilization is in 100-200 µL of DI water. Store at -20 °C immediately upon arrival.

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Protein Information

Intact Protein Formula C₆₄₇₂ H₉₉₄₀ N₁₆₉₈ O₂₀₀₈ S₅₂

Average MW = 145,329.7

17 Disulfide Bonds
N-terminus of each heavy chain has a fixed Pyroglutamic acid from Q
One N-linked glycosylation on each heavy chain. Total Number of Glycoforms: 4 (G0, G0F, G1F, G2F)
Disclaimer: I/L are exchangeable

Sequence: Light Chain Information

DVLMTQTPLS LPVSLGDQAS ISCRSSQYIV  HSNGNTYLEW
YLQKPGQSPK LLIYKVSNRF    SGVPDRFSGS GSGTDFTLKI
SRVEAEDLGV YYCFQGSHVP LTFGAGTKLE IKRADAAPTV
SIFPPSSEQL   TSGGASVVCF LNNFYPKDIN VKWKIDGSER
QNGVLNSWTD  QDSKDSTYSM SSTLTLTKDE
YERHNSYTCE ATHKTSTSPI   VKSFNRNEC

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Sequence: Heavy Chain Information

*QVQLKESGPG LVAPSQSLSI TCTVSGFSLL GYGVNWVRQP PGQGLEWLGM IWGDGSTDYN SALKSRITIS KDNSKSQVFL KMNSLQTDDT AKYYCTRAPY GKQYFAYWGQ GTLVTVSAAK TTPPSVYPLA PGSAAQTDSM VTLGCLVKGY FPEPVTVTWN SGSLSSGVHT FPAVLQSDLY TLSSSVTVPS STWPSETVTC NVAHPASSTK VDKKIVPRDC GCKPCICTVP EVSSVFIFPP KPKDVLTITL TPKVTCVVVD ISKDDPEVQF SWFVDDVEVH TAHTQPREEQ FNSTFRSVSE LPIMHQDWLN GKEFKCRVNS AAFPAPIEKT ISKTKGRPKA PQVYTIPPPK EQMAKDKVSL TCMITDFFPE DITVEWQWNG QPAENYKNTQ PIMDTDGSYF VYSKLNVQKS NWEAGNTFTC SVLHEGLHNH HTEKSLSHSP G

* pyroglutamic acid from Q

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Intact mAb Mass Check Standard

Fully Characterized Monoclonal Antibody Standard

Protein Information

Sequence: Light Chain Information

Sequence: Heavy Chain Information

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