Using selectivity to avoid matrix effects
One method to avoid potential phospholipid coelution and therefore potential matrix effects is to change the chromatographic selectivity of a separation. In RPLC, relatively hydrophobic phospholipids require high percentages of organic solvent to elute from the column. Thus, hydrophobic compounds of interest are prone to coelute with these lipid interferences and experience matrix effects. UPC2 utilizes supercritical carbon dioxide as the major mobile phase, resulting in vast selectivity differences when compared to standard RPLC in bioanalysis.
