Oligonucleotide Separation Technology (OST) columns contain second-generation hybrid-silica BEH Technology™ particles functionalized with C18. The separation of detritylated synthetic oligonucleotide samples is based on the well-established method of ion-pair, reversed-phase chromatography.
This chemistry delivers exceptional sample resolution and superior column life. In addition, Waters manufacturing and quality control testing procedures help ensure consistent batch-to-batch and column-to-column performance regardless of application demands.
The availability of 1.7 μm UPLC® technology or 2.5 μm HPLC particles in various column dimensions gives you the flexibility to meet various lab-scale isolation or analysis needs.
Features:
Column | Approx. Mass Load (µmoles)** | Flow Rate (mL/min) |
2.1 x 50 | 0.04 | 0.2 |
4.6 x 50 | 0.20 | 1.0 |
10.0 x 50 | 1.00 | 4.5 |
19.0 x 50* | 4.00 | 16.0 |
30.0 x 50* | 9.00 | 40.0 |
50.0 x 50* | 25.0 | 110.0 |
* OST Custom Column
** Values are only approximates and vary depending on oligonucleotide length, base composition, and “heart-cutting” fraction collection method used.