Oligonucleotide Separation Technology (OST) columns contain second-generation hybrid-silica BEH Technology™ particles functionalized with C18. The separation of detritylated synthetic oligonucleotide samples is based on the well-established method of ion-pair, reversed-phase chromatography.
This chemistry delivers exceptional sample resolution and superior column life. In addition, Waters manufacturing and quality control testing procedures help ensure consistent batch-to-batch and column-to-column performance regardless of application demands.
The availability of 1.7 μm UPLC® technology or 2.5 μm HPLC particles in various column dimensions gives you the flexibility to meet various lab-scale isolation or analysis needs.
|Column||Approx. Mass Load|
|2.1 x 50||0.04||0.2|
|4.6 x 50||0.20||1.0|
|10.0 x 50||1.00||4.5|
|19.0 x 50*||4.00||16.0|
|30.0 x 50*||9.00||40.0|
|50.0 x 50*||25.0||110.0|
* OST Custom Column
** Values are only approximates and vary depending on oligonucleotide length, base composition, and “heart-cutting” fraction collection method used.