Oligonucleotides Separation Technology Columns

Oligonucleotide Separation Technology (OST) columns contain second-generation hybrid-silica BEH Technology™ particles functionalized with C₁₈. The separation of detritylated synthetic oligonucleotide samples is based on the well-established method of ion-pair, reversed-phase chromatography.

This chemistry delivers exceptional sample resolution and superior column life. In addition, Waters manufacturing and quality control testing procedures help ensure consistent batch-to-batch and column-to-column performance regardless of application demands.

The availability of 1.7 μm UPLC^® technology or 2.5 μm HPLC particles in various column dimensions gives you the flexibility to meet various lab-scale isolation or analysis needs.

Features:

Scalable reversed-phase HPLC columns for lab-scale purifications
Increased sample through-put with maintained component resolution with UPLC Technology
LC/UV and LC/MS methods for enhanced quantitative and qualitative analyses
Exceptional column life using Waters patented Ethylene-Bridged (BEH Technology) Hybrid particles

[ XBridge OST C18 Column Selection Guide for Detritylated Olig ]
Separation of 5-25 mer Detritylated Oligodeoxythymidine Ladd

Column	Approx. Mass Load (µmoles)**	Flow Rate (mL/min)
2.1 x 50	0.04	0.2
4.6 x 50	0.20	1.0
10.0 x 50	1.00	4.5
19.0 x 50*	4.00	16.0
30.0 x 50*	9.00	40.0
50.0 x 50*	25.0	110.0

* OST Custom Column

** Values are only approximates and vary depending on oligonucleotide length, base composition, and “heart-cutting” fraction collection method used.

Oligonucleotides Separation Technology Columns

[ XBridge OST C18 Column Selection Guide for Detritylated Olig ]

Separation of 5-25 mer Detritylated Oligodeoxythymidine Ladd

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