Note: This item is also available with one vial the BEH200 SEC Protein Standard at no additional cost (p/n: 176004325).
This guard column is compatible with:
p/n: 186009163 XBridge Protein BEH SEC Column, 125Å, 2.5 µm, 7.8 mm X 150 mm
p/n: 186009164 XBridge Protein BEH SEC Column, 125Å, 2.5 µm, 7.8 mm X 300 mm
p/n: 176004326 XBridge Protein BEH SEC Column, 125Å, 2.5 µm, 7.8 mm X 150 mm w/ Standard
p/n: 176004327 XBridge Protein BEH SEC Column, 125Å, 2.5 µm, 7.8mm X 300 mm w/ Standard
Chemistry |
SEC |
Separation Mode |
Gel Filtration (aqueous) |
Particle Substrate |
Hybrid |
pH Range Min |
2.5 pH |
pH Range Max |
8 pH |
Temperature Limits |
60 C |
Maximum Pressure |
4500 psi (310 Bar) |
Endcapped |
No |
Bonding Technology |
Diol (OH) |
Silanol Activity |
Low |
Molecular Weight Range Min |
10000 |
Molecular Weight Range Max |
450000 |
Particle Size |
2.5 µm |
Endfitting Type |
Parker-style |
Pore Size |
200 Å |
QC Tested |
Protein |
Format |
Online Column |
Packing Solvent |
20% methanol in water |
Surface Area |
220 |
System |
HPLC, UHPLC, UPLC |
Particle Technology |
BEH |
Technique |
LC |
USP Classification |
L20, L33, L59 |
Inner Diameter |
7.8 mm |
Length |
30 mm |
Carbon Load |
12 % |
eCord |
No |
UNSPSC |
41115711 |
Application |
Protein |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
XBridge Protein BEH SEC Guard Column, 200 Å, 2.5 µm, 7.8 mm X 30 mm, 1/pk
Expand the efficiency and lifespan of your size-exclusion chromatography (SEC) applications with the XBridge Protein BEH SEC Guard Column, a short, pre-packed guard column designed to safeguard the analytical column and improve peak shape by removing contaminants from the lab equipment. It boasts a 200 Å pore size, a 2.5 µm particle size, and dimensions of 7.8 mm X 30 mm, making it an ideal choice for liquid chromatography-mass spectrometry (LC-MS) applications.
The XBridge Protein BEH SEC Guard Column offers numerous advantages, starting with its ability to protect the analytical column from particulates and other contaminants that can cause damage and shorten its lifespan. By ensuring the removal of contaminants, it enhances peak shape and resolution, resulting in superior chromatographic performance. Columns like the XBridge Protein BEH SEC Column, 200 Å, 2.5 µm, 7.8 mm X 150 mm, 1/pk and BEH200 SEC Protein Standard Mix are the optimal fit for the tool.
The inclusion of the XBridge Protein BEH SEC Guard Column in your chromatographic workflow significantly increases the lifespan of the analytical column by shielding it from potential damage, thus promoting cost-effectiveness and sustained efficiency.
As a versatile and reliable column, the XBridge Protein BEH SEC Guard Column finds applicability across diverse fields such as proteomics, biomarker discovery, drug development, clinical research, and forensics, where it efficiently separates proteins and peptides, identifies and quantifies biomarkers, screens drug candidates, and aids in disease diagnosis and chemical identification.
Packed in a guard cartridge that is compatible with a wide range of LC systems, the XBridge Protein BEH SEC Guard Column grants you the flexibility to seamlessly integrate it into your existing workflow, optimizing your chromatographic processes. It features a chemically stable, end-capped diol-bonded phase, offering exceptional stability across the pH spectrum from 2 to 11, ensuring reliable performance throughout your analyses.
For those seeking a reliable and efficient guard column for SEC applications, the XBridge Protein BEH SEC Guard Column stands out as an excellent option, offering versatility and reliability across a wide range of analytical tasks. Embrace the ease of use and maintenance offered by the XBridge Protein BEH SEC Guard Column, ensuring a smooth and efficient chromatographic experience while safeguarding your analytical column and elevating your SEC applications to new heights of performance and longevity.
How often should I replace XBridge Protein BEH SEC Guard Column?
The frequency with which you need to replace XBridge Protein BEH SEC Guard Column will depend on the type of samples you are analyzing and the frequency with which you are using your LC system. However, as a general rule of thumb, you should replace the guard column every 50-100 injections.