XBridge Protein BEH SEC, 200 Å Column, containing 2.5um particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns. They are best suited to separate proteins ranging from 10,000 – 450,000 Daltons. Note: This PN iincludes one vial the BEH200 SEC Protein Std at no additional cost (PN 186006518).
Chemistry |
SEC |
Separation Mode |
Gel Filtration (aqueous) |
Particle Substrate |
Hybrid |
pH Range Min |
2.5 pH |
pH Range Max |
8 pH |
Temperature Limits |
60 C |
Maximum Pressure |
6,500 psi (448 Bar) |
Endcapped |
No |
Bonding Technology |
Diol (OH) |
Silanol Activity |
Low |
Molecular Weight Range Min |
10000 |
Molecular Weight Range Max |
450000 |
Particle Size |
2.5 µm |
Endfitting Type |
Parker-style |
Pore Size |
200 Å |
QC Tested |
Protein |
Format |
Online Column |
Packing Solvent |
20% methanol in water |
Surface Area |
220 |
System |
UPLC |
Particle Technology |
BEH |
Technique |
LC |
USP Classification |
L33 |
Inner Diameter |
7.8 mm |
Length |
150 mm |
Carbon Load |
12 % |
eCord |
No |
UNSPSC |
41115711 |
Application |
Protein |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
XBridge Protein BEH SEC Column, 200 Å, 2.5 µm, 7.8 mm X 150 mm, 1/pk and BEH200 SEC Protein Standard Mix
The XBridge Protein BEH SEC Column and BEH200 SEC Protein Standard Mix were created to meet even the most rigorous chromatographic requirements. You may achieve unique, mobile-phase pH stability with the XBridge Protein BEH SEC Column and BEH200 SEC Protein Standard Mix, which helps to extend column lifetime. The XBridge Protein BEH SEC Column and BEH200 SEC Protein Standard Mix have been designed to increase productivity in your lab, and these multi-purpose columns can be used to construct a quality control technique or to develop a cutting-edge LC-MS assay.
You will have outstanding column reliability in your system with the use of the XBridge Protein BEH SEC Column, which ensures the ruggedness of assays and provides unrivaled peak particle shape and capacity with exceptional efficiency. A vial of the BEH200 SEC Protein Standard Mix is included with the XBridge Protein BEH SEC Column. It is advised that the BEH200 SEC Protein Standard Mix be used as a benchmark for column and system performance.
The XBridge Protein BEH SEC Column is based on Ethylene Bridged Hybrid (BEH)-based particle technology with dial-bonded surface coating and is recommended for separating proteins in the 10,000–450,000 Daltons range. Waters equipment undergoes various examinations and individual tests in our dedicated facilities to ensure you obtain the highest quality products for your laboratory and that you can rely on the equipment to deliver precision-focused results. These facilities adhere to tight industrial practices to ensure that the highest quality standards are met and equipment is manufactured in accordance with ISO9001 and cGMP guidelines.
Browse our website to see our full line of XBridge columns and to shop for lab equipment that meets your specific requirements. If you require assistance with any equipment or have any questions, please contact a member of our global staff.
You may also want to explore TruView LCMS Certified Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk; TruView LCMS Certified vials are produced following tightly controlled manufacturing processes and handling procedures. They are tested for cleanliness by mass spectrometers and for low analyte adsorption by ACQUITY/MS/MS.
Under What pH Range Can The XBridge Protein BEH SEC Column and BEH200 SEC Protein Standard Mix Operate?
The XBridge Protein BEH SEC Column and BEH200 SEC Protein Standard Mix operate ideally within the 2.5 - 8 pH range, a temperature limit of 60 C, and a maximum pressure of 6,500 psi (448 Bar). However, we don’t recommend using higher pH, pressure, or temperatures than these range as it may impact the performance and lifetime of your column.