Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Temperature Limits |
45 C |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
Yes |
Silanol Activity |
High |
Particle Shape |
Spherical |
Particle Size |
5 µm |
Endfitting Type |
Waters |
Pore Size |
80 Å |
Format |
OBD Prep Column |
Surface Area |
220 |
System |
HPLC Preparative |
Technique |
LC/MS, LC |
USP Classification |
L1 |
Inner Diameter |
19 mm |
Length |
250 mm |
Carbon Load |
12 % |
UNSPSC |
41115709 |
Brand |
Spherisorb |
Product Type |
Preparative Columns |
Units per Package |
1 pk |
Spherisorb C18 OBD Prep Column, 80Å, 5 µm, 19 mm X 250 mm, 1/pk
Achieve exceptional flexibility in optimizing methods and reducing solvent consumption by using the Spherisorb C18 OBD Prep Column. The lab equipment is a general-purpose, silica-based, reversed-phase C18 column that contains packing with intermediate ligand density with a specified carbon load of 11.5%. The preparatory column has been designed to be used for general purposes and is a durable and high-efficiency chromatographic equipment, able to cater to a wide range of applications.
You can depend on the Spherisorb C18 OBD Prep Column to meet the quality standards required for liquid chromatography. The preparatory column is manufactured under the strictest cGMP, ISO 9002 conditions in the dedicated Waters chromatography chemistry manufacturing plant, where the silica-based and polymeric chromatography materials are synthesized and packed into columns and solid-phase extraction devices. Through this diligence, you are ensured the highest quality lab equipment that delivers consistent and reliable analysis results. By adding the Spherisorb C18 OBD Prep Column to your lab, you benefit from the most critical performance features required for exceptional performance: column efficiency, peak symmetry, column lifetime, and column-to-column reproducibility.
The scientists at Waters have developed the Spherisorb C18 OBD Prep Column after extensive research that was conducted to understand and address the effects of all process parameters such as slurry solvent choice, packing pressure time, temperature, flow rate, and slurry volume. The results of this extensive work are reflected in the column packing processes used in its manufacture to ensure that the lab equipment delivers maximum performance every time. This means that you get the best column stability and highest efficiency along with the best peak symmetry.
The Spherisorb C18 OBD Prep Column uses a high-quality bonded phase, which enables many different and unique separation selectivities. You have a choice to select a column configuration based on your sensitivity needs, sample capacity, mobile phase, and budgetary needs. You can find out the entire range of options available in the Spherisorb brand through our website which also allows you to shop for lab equipment. You may also be interested in the Spherisorb ODS2 Column, 80Å, 5 µm, 4.6 mm X 250 mm, 1/pk.
Why Does The Carbon Load Of My Column Matter?
The carbon load of any given column is given in a percentage, and it refers to the carbon content of the silica bonded stationary phase. For the most part, a higher carbon load of between 18 and 25% will bring about a more hydrophobic surface, as well as one that is more resistant to high pH. However, a higher carbon load does not always mean the results will be better, so it’s important to select a piece of lab equipment that suits your specific needs and carbon content requirements.