ACQUITY UPLC M-Class Columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation for biomarker discovery and protein and peptide identification and characterization. All M-Class columns are designed for 15 kpsi (1,000 bar) operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity.
This specific offering contains Waters patented BEH Technology 300 Å pore, C18, 1.7um particles. BEH technology offers the highest retentivity for basic compounds and provides excellent peak shape at elevated pH mobile phases and is suitable for separations up to 80°C. BEH is the universal column choice for a variety of compounds.
Each batch is specifically QC tested using a gradient separation of a tryptic digest of cytochrome c to before being QC accepted as a Peptide Separation Column.
Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
10 pH |
Maximum Pressure |
15000 psi (1034 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
Valco(male)|360um Open |
Pore Size |
300 Å |
QC Tested |
Peptide |
Format |
Column |
Surface Area |
90 |
System |
Nano/Micro |
Particle Technology |
BEH |
USP Classification |
L1 |
Inner Diameter |
75 µm |
Length |
100 mm |
Carbon Load |
12 % |
UNSPSC |
41115709 |
Application |
Peptide |
Brand |
ACQUITY UPLC M-Class |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY UPLC M-Class Peptide BEH C18 Column, 300Å, 1.7 µm, 75 µm X 100 mm, 1/pk
Add the ACQUITY UPLC M-Class Peptide BEH C18 Column to your lab because it is made for liquid chromatographic separation at the nano- to microscale. For the identification and characterization of proteins as well as the development of biomarkers, the lab equipment provides the greatest levels of sensitivity, resolution, and repeatability. The nanoACQUITY UPLC® Systems are compatible with the lab equipment.
The right connections must be made to reduce peak tailing and inefficient operation in order to achieve optimal performance. This is significant because, in contrast to conventional flow chromatography, capillary-flow systems may necessitate greater attention to detail for good day-to-day operation. To provide exceptional performance, the lab equipment listed here incorporates patented Waters BEH Technology 300 pore, C18, 1.7um particles. Before being QC recognized as a Peptide Separation Column, each batch of the packing material used in the column's production is evaluated utilizing a gradient separation of a tryptic digest of cytochrome C.
The ACQUITY UPLC M-Class Peptide BEH C18 Column is produced in accordance with stringent specifications and fritted using cutting-edge frit technology. Due to these strict requirements and the chemistry of the column, it can endure high pressures. Moreover, it can manage pressure changes during injection quite well, which results in improved longevity. Each ACQUITY UPLC M-Class Column is individually inspected to guarantee that it meets rigorous quality standards, ensuring that you only receive high-quality equipment. In a closely watched and rigorously regulated environment, the column is produced in Waters' ISO 9001-certified plant.
Visit our website to learn more about our wide range of products and which ones complement the ACQUITY UPLC M-Class Column the best. You may shop for lab equipment using it as well.
We recommend that you also learn more about the MassPREP Enolase Digestion Standard. The standard is helpful in a number of different applications, such as evaluating the performance of HPLC columns, high-performance liquid chromatography, and mass spectrometry equipment used for the analysis of this family of compounds.
What Carbon Load Is Carried By The ACQUITY UPLC M-Class Peptide BEH C18 Column?
This particular ACQUITY UPLC M-Class Peptide BEH C18 Column carries a carbon load of 12 %.
What Is The Maximum Pressure That The ACQUITY UPLC M-Class Peptide BEH C18 Column Can Tolerate?
The ACQUITY UPLC M-Class Peptide BEH C18 Column listed here is able to tolerate a maximum of 15000 psi (1034 Bar) pressure.