SKU: 186007483
ACQUITY UPLC M-Class Peptide BEH C18 Column, 130Å, 1.7 µm, 75 µm X 200 mm, 1/pk

ACQUITY UPLC M-Class Peptide BEH C18 Column | 186007483


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Product Description

ACQUITY UPLC M-Class Columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation, offering the highest possible sensitivity, resolution and reproducibility for biomarker discovery and protein identification and characterization. As the 75µm to 180µm ID columns are fritted with a novel frit technology, they are able to withstand 15kpsi operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity. In order to achieve maximum performance, it is important to ensure that proper connections are made to minimize peak tailing and poor efficiency.  This specific offering contains Waters patented BEH Technology 130A pore, C18, 1.7um particles. Each batch is specifically QC tested using a gradient separation of a tryptic digest of cytochrome c to before being QC accepted as a Peptide separation column. 

Specifications

  • Chemistry

    C18

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Hybrid

  • pH Range Min

    1 pH

  • pH Range Max

    10 pH

  • Maximum Pressure

    15000 psi (1034 Bar)

  • Endcapped

    Yes

  • Silanol Activity

    Low

  • Particle Shape

    Spherical

  • Particle Size

    1.7 µm

  • Endfitting Type

    Valco(male)|360um Open

  • Pore Size

    130 Å

  • QC Tested

    Peptide

  • Format

    Column

  • Surface Area

    185

  • System

    Nano/Micro

  • Particle Technology

    BEH

  • USP Classification

    L1

  • Inner Diameter

    75 µm

  • Length

    200 mm

  • Carbon Load

    18 %

  • UNSPSC

    41115709

  • Application

    Peptide

  • Brand

    ACQUITY UPLC M-Class

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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ACQUITY UPLC M-Class Peptide BEH C18 Column, 130Å, 1.7 µm, 75 µm X 200 mm, 1/pk

The ACQUITY UPLC M-Class Peptide BEH C18 Column is designed to provide optimal performance for nano- to micro-scale liquid chromatographic separation. The ACQUITY UPLC M-Class Peptide BEH C18 Column also has a range of additional features, such as the highest possible sensitivity, resolution, and reproducibility for biomarker discovery and protein identification and characterization, making it a must-have piece of lab equipment.

The ACQUITY UPLC M-Class Peptide BEH C18 Column is manufactured under tightly controlled standards and fritted with a novel frit technology. The column is manufactured in Waters ISO 9001 certified plant in a carefully monitored and strictly controlled environment. The strict manufacturing conditions, combined with the column’s chemistry, enable the ACQUITY UPLC M-Class Peptide BEH C18 Column to be able to withstand high pressures of up to 15,000 psi (1,000 bar) and also make it capable of handling pressure fluctuations during injection extremely well. These features of the ACQUITY UPLC M-Class Peptide BEH C18 Column provide the column with superior longevity.

In an effort to ensure the column provides maximum pressure, it is highly recommended and important to ensure that proper connections are made to minimize peak tailing and poor efficiency. The specific lab equipment in this listing contains Waters patented BEH Technology 130Å pore, C18, 1.7um particles, which enables the Column to deliver superior performance. Each batch of the packing material used in the manufacturing of the ACQUITY UPLC M-Class Peptide BEH C18 Column is extensively QC tested using a gradient separation of a tryptic digest of cytochrome c before being QC accepted as a Peptide separation column.

If you want to review the additional collection of lab equipment from Waters, you may review our catalog or browse through our website from where you can shop for lab equipment to fulfill all your laboratory needs as applicable.

You may also want to check out TruView LCMS Certified Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk, which have been tested for cleanliness by mass spectrometers and for low analyte adsorption by ACQUITY/MS/MS.

What Considerations Must Be Taken Into Account For Sample Preparation?

Samples may contain approved digestion buffers and residual reagents, but it is crucial to ensure that they do not contain additional reagents, denaturants, detergents, lipids, or particulates. The presence of undigested protein in significant amounts per injection can have a negative impact on column lifetimes, depending on the injected quantity and column diameter. To mitigate this, it is essential to adhere to proper sample preparation protocols to maintain the integrity and longevity of the column.