Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Temperature Limits |
45 C |
Maximum Pressure |
18000 psi (1240 Bar) |
Endcapped |
Yes |
Bonding Technology |
T3 |
Silanol Activity |
Medium |
Particle Shape |
Spherical |
Particle Size |
1.8 µm |
Endfitting Type |
Parker-style |
Pore Size |
100 Å |
Format |
Column |
Surface Area |
230 |
System |
UHPLC, UPLC |
Particle Technology |
HSS |
USP Classification |
L1 |
Inner Diameter |
1 mm |
Length |
50 mm |
Carbon Load |
11 % |
eCord |
Yes |
UNSPSC |
41115709 |
Brand |
ACQUITY UPLC |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY UPLC HSS T3 Column, 100Å, 1.8 µm, 1 mm X 50 mm, 1/pk
The sorbent used in the ACQUITY UPLC HSS T3 Column uses a universal, silica-based bonded phase that is compatible with 100% aqueous mobile phase. The reliable column should be your first choice for the retention and separation of polar and non-polar compounds. The ACQUITY UPLC HSS T3 Column exhibits ultra-low MS bleed and is fully scalable and transferable to HPLC separations.
The ACQUITY UPLC HSS T3 Column is manufactured with the advanced T3 bonding process, which uses a trifunctional C18 alkyl phase bonded at a ligand density that promotes polar compound retention and aqueous mobile phase compatibility. When compared to the traditional trimethyl silane (TMS) end-capping, the proprietary T3 end-capping process is much more effective. The combination of T3 bonding and this unique end-capping gives you the superior polar compound retention and aqueous compatibility edge over the traditional columns, as well as delivering enhanced column performance, lifetime, peak shape, and stability. The ACQUITY UPLC HSS T3 Column uses the reversed-phase HPLC and UPLC technologies to maintain the industry leader status.
The ACQUITY UPLC HSS T3 Column uses the Waters HSS (High Strength Silica) particle technology, which was created to withstand UPLC pressures using a mechanically tolerant, silica-based material. The HSS incorporates a particle technology which is an innovative synthetic process that significantly increases the mechanical stability of silica while maintaining pore volumes similar to that of HPLC silica-based materials. This gives you increased retentivity compared to hybrid particle technology, and the HSS serves as the ideal substrate to create stationary phases that offer alternate selectivity.
Leveraging the balanced retention of polar and hydrophobic molecules and opting for the ACQUITY UPLC HSS T3 Column is a great choice when you need to develop separations of polar and non-polar compounds.
Shop for lab equipment such as the listing here and find all related products that work well with the ACQUITY UPLC HSS T3 Column using our website or catalog.
We recommend that you also purchase the TruView LCMS Certified Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk so you can easily store your analytes in an inert environment.
What Is The Recommended pH Range For The ACQUITY UPLC HSS T3 Column?
The ACQUITY UPLC HSS T3 Column works well between a pH range of 2-8.