Line drawing of a column

SKU: 186000281
Symmetry C4 Column, 300 Å, 3.5 µm, 4.6 mm x 75 mm, 1K - 30K, 1/pk

Symmetry C4 Column | 186000281


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Product Description

Primarily supports legacy methods. Symmetry columns give you confidence in the long-term compliance of your HPLC methods. No need to compromise on peak shape, selectivity, column-to-column and batch-to-batch reproducibility, or other critical performance characteristics. Available in a variety of particle sizes, chemistries and dimensions.

Specifications

  • Chemistry

    C4

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Silica

  • pH Range Min

    2 pH

  • pH Range Max

    8 pH

  • Maximum Pressure

    6000 psi (415 Bar)

  • Endcapped

    Yes

  • Silanol Activity

    Low

  • Molecular Weight Range Min

    1000

  • Molecular Weight Range Max

    30000

  • Particle Shape

    Spherical

  • Particle Size

    3.5 µm

  • Endfitting Type

    Waters

  • Pore Size

    300 Å

  • Format

    Column

  • Surface Area

    110

  • System

    HPLC

  • USP Classification

    L26

  • Inner Diameter

    4.6 mm

  • Length

    75 mm

  • Carbon Load

    2.8 %

  • UNSPSC

    41115709

  • Application

    Protein

  • Brand

    Symmetry

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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Symmetry C4 Column, 300Å, 3.5 µm, 4.6 mm X 75 mm, 1K - 30K, 1/pk

With the Symmetry C4 Column, you can separate physiologically active peptides with better peak shape and peak capacity than you can with traditional columns with similar chemistry. The Symmetry C4 Column performs better while concurrently separating hydrophilic and hydrophobic peptides, as well as neutral and strongly basic peptides.

Symmetry columns continue to set the standard for HPLC drug assays, giving you confidence in the long-term compliance of your HPLC methods. No need to compromise on peak shape, selectivity, column-to-column and batch-to-batch reproducibility, or other critical performance characteristics. Available in a variety of particle sizes, chemistries, and dimensions. You can shop for lab equipment using our website.

Utilize the Symmetry C4 Column, which has been created to become the industry standard for HPLC drug assays, to have trust in the long-term compliance of your HPLC techniques. Critical analysis can be carried out using the lab equipment without sacrificing peak shape, selectivity, column-to-column and batch-to-batch consistency, or other crucial performance features. The wide-pored, reversed-phase Symmetry C4 Column is a popular option in the pharmaceutical business because it provides HPLC assays that are sensitive, reliable, and tough. Large peptides and proteins are best suited to it because of its C4 chemistry.

The Symmetry C4 Column will perform better in both high- and low-mass situations. For mass spectrometry applications, low TFA mobile phases like 0.1 percent formic acid are advised. No matter who or where utilizes the procedure, the analytical column gives the optimum answer for validation compliance.

You get the sharpest peaks for biomolecules and more peak capacity for intricate tryptic maps with the Symmetry C4 Column. This is made feasible by the use of ultrapure substances that are used to produce silica. The outcome is an analytical column with a very low silanol activity and high purity. Additionally, the lab equipment is packed to the best efficiency and reproducibility of any product on the market, offering you better precision for impurity and stability assays, more accurate quantitation, and adaptability to more robust procedures.

We recommend that you store your samples in the TruView LCMS Certified Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk.

What Is The pH Limitation Of The Symmetry C4 Column?

The Symmetry C4 Column listed here is able to function well across the 2-8 pH range.

What Is The Maximum Pressure Recommended For The Symmetry C4 Column?

The recommended maximum pressure for the Symmetry C4 Column listed here is 6000 psi (415 Bar).