ACQUITY UPLC Columns

ACQUITY UPLC Columns

Increase speed, sensitivity, and resolution, without compromising data integrity

Increase speed, sensitivity, and resolution, without compromising data integrity

Increasing sample throughput, achieving unmatched resolution, and improving analyte sensitivity are priorities for your separation goals. With Waters ACQUITY UPLC Columns, you can meet all your method requirements and substantially reduce your analysis time and cost-per-sample. 

Waters ACQUITY UPLC Columns’ sub-2-μm particle chemistries enable chromatographers to harness the separation efficiency improvements at increased flow rates of ultra-performance liquid chromatography (UPLC) technology, thereby improving analyte resolution and sensitivity as well as business productivity and profitability.  


Mitigate unwanted interactions between analytes and metal ions associated with stainless-steel column hardware with ACQUITY Premier Columns. 

Specifications

Overview

  • Best-in-class column efficiency for superior separation speed, sensitivity, and resolution
  • 8 UPLC particles of various substrate technologies, including Ethylene Bridged Hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH)
  • 22 stationary phases, including C18, Phenyl-Hexyl, C8, Embedded-Polar, C4, HILIC, Amide, Diol, Cyano, and PFP
  • 7 application-specific UPLC Column chemistries for SEC, proteins, peptides, oligonucleotides, and glycan analysis
  • More than 50 different column dimensions and configurations, with internal column diameters ranging from 1.0–4.6 mm and column lengths ranging from 30–300 mm
  • Prolonged column lifetimes with optional VanGuard Cartridges specifically designed using ACQUITY stationary phases

Recommended Use: For improved speed, sensitivity, and resolution in methods development.


ACQUITY UPLC BEH Column Characteristics

ACQUITY UPLC BEH Column Characteristics

ACQUITY UPLC BEH C18 Columns
  • Universal C18 column
  • Suitable for a diverse range of analytes
  • High efficiency
  • Industry-leading mobile phase pH 1-12 and temperature compatibility
ACQUITY UPLC BEH C8 Columns
  • Lower hydrophobicity and retention than a C18 column
  • Suitable for a diverse range of analytes
  • High efficiency
  • Industry-leading mobile phase pH 1-12 and temperature compatibility
ACQUITY UPLC BEH Shield RP18 Columns
  • Contains an embedded polar group combined with the hydrophobicity of C18
  • Complementary selectivity to a C18 column
  • Enhanced peak shape for basic compounds
  • Compatible with 100% aqueous mobile phases
  • High efficiency
ACQUITY UPLC BEH Phenyl Columns
  • Industry leading chemical stability
  • Suitable for a diverse range of analytes
  • High efficiency
  • Complimentary selectivity to straight-chain alkyl phases
ACQUITY UPLC BEH HILIC Columns
  • Improves the retention of very polar basic analytes
  • Complementary selectivity to reversed phase
  • High efficiency
  • Exceptional chemical stability and peak shape compared to silica-based HILIC stationary phases
ACQUITY UPLC BEH Amide Columns
  • Designed to retain highly polar analytes that are too polar to retain by reversed phase
  • Exceptional chemical stability at high pH and high temperature
  • Ideal for sugar/carbohydrate analysis
  • Exceptional retention of polar analytes spanning a wide range of polarity

ACQUITY UPLC CSH Column Characteristics

ACQUITY UPLC CSH Column Characteristics

ACQUITY UPLC CSH C18 Columns
  • Universal column choice for UV and MS applications
  • Suitable for a broad range of compounds
  • Alternate selectivity to BEH C18
  • Exceptional peak shape and increased load capacity
  • Suited for basic compounds at low pH
ACQUITY UPLC CSH Fluoro-Phenyl Columns
  • Exceptional selectivity for positional isomers, halogenated compounds and polar compounds
  • Enhanced retention of acidic compounds compared to traditional PFP-bonded stationary phases
ACQUITY UPLC CSH Phenyl-Hexyl Columns
  • Complementary selectivity to straight-chain alkyl phases
  • Exceptional peak shape for basic compounds under low- and high-pH conditions
  • Increased retention for acidic compounds at low pH

ACQUITY UPLC HSS Column Characteristics

ACQUITY UPLC HSS Column Characteristics

ACQUITY UPLC HSS T3 Columns
  • Ideal for the enhanced retention of polar and non-polar compounds
  • Compatible with 100% aqueous mobile phases
  • Mechanically stable at elevated pressures
ACQUITY UPLC HSS C18 Columns
  • General purpose silica-based C18 chemistry
  • Suitable for a broad range of compounds
  • Provides exceptional peak shapes and low pH stability
  • Delivers increased retention compared to hybrid organic/inorganic C18 columns
ACQUITY UPLC HSS C18 SB Columns
  • Low coverage silica-based C18 chemistry
  • Provides alternate selectivity for compounds impacted by silanophilic interactions
  • Increased silanol activity results in greater retention of basic compounds while reducing the retention of non-basic analytes
  • Provides exceptional peak shape and imparts Selectivity for Bases at low pH (SB)
ACQUITY UPLC HSS PFP Columns
  • Pentafluorophenyl (PFP) chemistry
  • Industry-leading reproducibility and peak shape
  • Ideally suited for planar aromatic compounds, positional isomers, halogenated compounds and polar analytes
ACQUITY UPLC HSS Cyano Columns
  • Provides low hydrophobicity and unique selectivity compared to straight-chain-alkyl columns
  • Ultra-stable retention
  • Exceptional peak shape and reproducibility under low- to mid-pH conditions
  • Compatible with both reversed-phase and normal-phase techniques

ACQUITY UPLC Application-Specific Column Characteristics

ACQUITY UPLC Application-Specific Column Characteristics

ACQUITY UPLC Glycan Columns
  • Suited for the HILIC analysis of neutral and acidic glycans
ACQUITY UPLC Oligonucleotide Columns
  • Suited for the analysis of detritylated oligonucleotides using ion-pair, reversed-phase chromatography
ACQUITY UPLC Peptide Columns
  • Suited for the reversed-phase analysis of hydrophobic and hydrophilic peptides at low and high pH
ACQUITY UPLC Protein Columns
  • Suited for the separation of proteins by size exclusion or by reversed-phase chromatography

Get superior peak shape for basic analytes

Reversed-phase bonded phases typically have poor peak shape for basic compounds when using formic acid, even at analytical mass loads. However, ACQUITY UPLC CSH Columns are the exception. These rugged UPLC Columns provide superior peak shape for basic analytes with formic acid or other low ionic-strength, acidic mobile phases. The controlled, low-level, positive surface charge bonded to the ethylene-bridged hybrid (BEH) particles provides excellent peak shape for bases—without the need for the use of ion-pairing reagents.


The ruggedness you want, the repeatability you need

ACQUITY UPLC BEH 1.7 μm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable separation scientists to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.


Achieve flexibility in method development

Chromatographic laboratories need to quickly and easily develop robust methods that are compatible with all modern chromatographic detection modes and transferrable to laboratories and sites that operate different LC system platforms. With ACQUITY UPLC CSH and XSelect Columns, method development scientists in all application areas can achieve a wide range of selectivities without compromising performance across attributes, including superior peak shape for basic compounds, low column bleeds, excellent batch-to-batch reproducibility, and high efficiency.



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Prolong analytical column lifetime while maintaining chromatographic performance with VanGuard Pre-columns and cartridges.

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