ACQUITY Premier Columns

ACQUITY Premier Columns

Stop looking and start seeing – no compromise required

Stop looking and start seeing – no compromise required

Reduce the risks associated with unpredictable chromatography with ACQUITY Premier Columns with MaxPeak High Performance Surfaces (HPS) Technology, which allow you to see and do more by reducing analyte/surface interactions.

With Waters ACQUITY Premier Columns, chromatographers can harness the separation efficiency improvements and increased flow rates of ultra-performance liquid chromatography (UPLC) technology, improving analyte resolution, sensitivity, business productivity, and profitability. As the next evolution in the ACQUITY UPLC Column family, ACQUITY Premier Columns are built on the industry-leading sub-2-µm particle technologies from Waters you trust.

ACQUITY Premier Column Family  ACQUITY Premier Columns

View the wide selection of ACQUITY UPLC sub-2-μm Column chemistries.

View the wide selection of ACQUITY UPLC sub-2-μm Column chemistries.

Specifications

Overview

  • Reduces column conditioning and passivation for higher productivity
  • Increases reproducibility from analysis to analysis, for more confidence in analytical results
  • Reduces non-specific absorption for increased sensitivity, improved peak shape, and easier analyte integration
  • Provides superior separation speed, sensitivity, and resolution with best-in-class column efficiency
  • 3 UPLC particles of various substrate technologies, including Ethylene Bridged Hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH)
  • Reversed-phase and HILIC stationary phases, including C18, Embedded-Polar, and Amide
  • Application-specific column chemistries for peptides, proteins, oligonucleotides, and glycan analysis
  • Prolonged column lifetimes with VanGuard FIT Columns, and easy-to-use, all-in-one guard/column design

Recommended Use: For increased sensitivity, improved peak shape, and less column equilibration time in methods development.


ACQUITY Premier Column Featured Technologies

ACQUITY Premier Column Featured Technologies

MaxPeak HPS Technology
  • Reduce analyte/surface interactions
  • Increase analyte recovery
  • Better peak shape
  • Increase sensitivity
VanGuard FIT*
  • All-in-one guard, plus column design
  • Achieve longer column lifetimes
  • Easy installation

* Specific to columns noted with VanGuard FIT

ACQUITY Premier BEH Column Characteristics

ACQUITY Premier BEH Column Characteristics

ACQUITY Premier BEH C18 Columns
  • Universal C18 Column
  • Suitable for a diverse range of analytes
  • High efficiency
  • Industry-leading mobile-phase pH 1–12 and temperature compatibility
ACQUITY Premier BEH Shield RP18 Columns
  • Contains an embedded-polar group combined with the hydrophobicity of C18
  • Complementary selectivity to a C18 column
  • Enhanced peak shape for basic compounds
  • Compatible with 100% aqueous mobile phases
  • High efficiency
ACQUITY Premier BEH Amide Columns
  • Designed to retain highly polar analytes that are too polar to retain by reversed-phase
  • Exceptional chemical stability at high pH and high temperature
  • Ideal for sugar/carbohydrate analysis
  • Exceptional retention of polar analytes spanning a wide range of polarity

ACQUITY Premier CSH Column Characteristics

ACQUITY Premier CSH Column Characteristics

ACQUITY Premier CSH C18 Columns
  • Universal column choice for UV and MS applications
  • Suitable for a broad range of compounds
  • Alternate selectivity to BEH C18
  • Exceptional peak shape and increased load capacity
  • Suited for basic compounds at low pH
ACQUITY Premier CSH Phenyl-Hexyl Columns
  • Complementary selectivity to straight-chain-alkyl phases
  • Exceptional peak shape for basic compounds under low- and high-pH conditions
  • Increased retention for acidic compounds at low pH

ACQUITY Premier HSS Column Characteristics

ACQUITY Premier HSS Column Characteristics

ACQUITY Premier HSS T3 Columns
  • Ideal for the enhanced retention of polar and non-polar compounds
  • Compatible with 100% aqueous mobile phases
  • Mechanically stable at elevated pressures

ACQUITY Premier Application-Specific Column Characteristics

ACQUITY Premier Application-Specific Column Characteristics

ACQUITY Premier Glycan Columns
  • Suited for the HILIC analysis of neutral and acidic glycans
ACQUITY Premier Oligonucleotide Columns
  • Suited for the analysis of detritylated oligonucleotides using ion-pair, reversed-phase chromatography
ACQUITY Premier Peptide Columns
  • Suited for the reversed-phase analysis of hydrophobic and hydrophilic peptides at low and high pH

Get superior peak shape for phosphorylated and carboxylated analytes

Phosphorylated and carboxylated analytes are metal sensitive and can readily adsorb to stainless steel surfaces, leading to poor peak shape, low recovery, and a reduction in sensitivity. ACQUITY Premier Columns improved peak shape for these analytes, making it easier for scientists to accurately integrate and quantify these troubling compounds.


Ruggedness you want, the repeatability you need

ACQUITY Premier Oligonucleotide Columns provide superior analyte peak shape, efficiency, and chemical stability for your oligo separations. ACQUITY Premier Oligonucleotide Columns are packed with BEH particle technology and able to operate at an elevated pH – ideal for oligo separations. The MaxPeak HPS Technology in ACQUITY Premier Oligonucleotide Columns improves the peak shape for oligos, making it easier to identify and quantify these analytes.


Achieve flexibility in method development

Chromatographic laboratories need to quickly and easily develop robust methods, but those methods need to be compatible with all modern chromatographic detection modes and transfer to laboratories and sites on different LC system platforms. With ACQUITY Premier Columns, method development scientists in all application areas can achieve a wide range of selectivities while ensuring high performance, including superior peak shape for basic compounds, low column bleeds, excellent batch-to-batch reproducibility, and high efficiency.



Resources

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Related

Discover how to achieve increases in LC efficiency, resolution, sensitivity, accuracy, speed, and reliability with ACQUITY sub-2-μm UPLC particle columns.

Ensure the successful development and commercialization of effective and safe glycosylated biotherapeutics. Waters offers a variety of robust, reproducible, complementary, information-rich analytical methods for glycan and glycoprotein separations.

Deliver exceptional sample resolution and superior column lifetime with Waters Oligonucleotide columns for DNA/RNA oligonucleotide separations. Each column is QC-tested to ensure batch-to-batch performance.

Characterize complex biomolecules with Waters reversed-phase, ion-exchange, hydrophilic-interaction, and size-exclusion chromatography columns for peptide separation applications such as the analysis and purification of synthetic peptides, the characterization of complex proteolytic digests, and bioanalysis.

Achieve better resolution of complex protein digests, exceptional glycopeptide resolution, and higher throughput with reversed-phase, ion-exchange, hydrophilic-interaction, and size-exclusion chromatography columns for peptide separation applications ranging from the analysis and purification of synthetic peptides to the characterization of complex biotherapeutic proteins.

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