Targeted Omics and Biomarker Quantification

Targeted Omics and Biomarker Quantification

Transition from biomarker discovery to verification and quantification of biomarkers using high-throughput, high-sensitivity quantitative LC-MS/MS solutions. With Waters Targeted Omics Method Library, you can quickly and easily implement precise targeted quantification methods for endogenous proteins, lipids, and metabolic biomarkers while supporting optimum assay sensitivity and robustness.

Transition from biomarker discovery to verification and quantification of biomarkers using high-throughput, high-sensitivity quantitative LC-MS/MS solutions. With Waters Targeted Omics Method Library, you can quickly and easily implement precise targeted quantification methods for endogenous proteins, lipids, and metabolic biomarkers while supporting optimum assay sensitivity and robustness.
Protein used as biomarker of kidney function
Protein used as biomarker of kidney function

Overview

Translating biomarker discoveries into practical, quantitative assays can be complex, requiring considerations for throughput, reproducibility, scalability, and sensitivity. As demand grows for targeted assays in proteomics, metabolomics, and lipidomics, method development, and implementation become increasingly time-intensive and challenging.

Waters streamlines targeted omics analysis with innovative LC-MS/MS solutions that enhance your laboratory’s biomarker quantification with precision and accuracy, accelerating results, and improving efficiency. The Waters Targeted Omics Library offers ready-to-use, downloadable methods, reducing development time, and enabling your lab to stay ahead in biomarker discovery—without overextending your resources. With Waters, you can:

  • Implement robust, sensitive quantitative biomarker assays with Xevo TQ-S micro and Xevo TQ Absolute Mass Spectrometers
  • Integrated LC solutions to meet your combined throughout and sensitivity requirments including nano-flow, micro-flow, or standards flow interface options
  • Minimize nonspecific binding in proteins and lipid marker assays with ACQUITY Premier UPLC System and columns and MaxPeak Premier consumables
  • Access Waters Targeted Omics Method Library with ready-to-use application notes, method files, and isotope-labeled standards for seamless LC-MS/MS implementation
  • Optimize proteomics workflows through direct integration with Skyline via the MassLynx Skyline Interface (MSI)
  • Run large sample cohorts efficiently to achieve statistical power
Download Infographic

Applications

Discover how Waters LC-MS/MS solutions tackle common challenges in peptides MRM selection, method optimization, and sensitivity in targeted proteomics assays. With robust, high-throughput, and high-sensitivity technologies, Waters supports efficient quantitative method development implementation for high quality quantitation of peptide biomarkers.

Discover how Waters LC-MS/MS solutions tackle common challenges in peptides MRM selection, method optimization, and sensitivity in targeted proteomics assays. With robust, high-throughput, and high-sensitivity technologies, Waters supports efficient quantitative method development implementation for high quality quantitation of peptide biomarkers.

Application Notes

Molecular structure of a peptide, sequence of amino acids

When measuring changes in specific lipids within complex samples, method development can be challenging. Discover how the Waters free-to-download LipidQuan-R methodologies streamline the implementation of high-performance LC-MS/MS assays, maximizing the benefits of Waters chromatography and mass spectrometry technologies for lipid quantification through minimized non-specific binding and optimized negative ion MS sensitivity.

When measuring changes in specific lipids within complex samples, method development can be challenging. Discover how the Waters free-to-download LipidQuan-R methodologies streamline the implementation of high-performance LC-MS/MS assays, maximizing the benefits of Waters chromatography and mass spectrometry technologies for lipid quantification through minimized non-specific binding and optimized negative ion MS sensitivity.

Application Notes

Cell membrane, lipid bilayer

Address the challenges of monitoring low-mass, highly polar endogenous metabolites with Waters targeted metabolomics solutions, including free-to-download MetaboQuan-R methodologies and high-performance, low-binding chemistries paired with advanced LC-MS/MS technologies.

Address the challenges of monitoring low-mass, highly polar endogenous metabolites with Waters targeted metabolomics solutions, including free-to-download MetaboQuan-R methodologies and high-performance, low-binding chemistries paired with advanced LC-MS/MS technologies.

Application Notes

Small molecules floating

Solutions

With Waters systems, streamline analysis and accelerate sample throughput with solutions tailored to the needs of your laboratory.

With Waters systems, streamline analysis and accelerate sample throughput with solutions tailored to the needs of your laboratory.

Absolute performance for quantitative analysis

Absolute performance for quantitative analysis
Xevo TQ Absolute Triple Quadrupole Mass Spectrometer

Expand your analytical capabilities with the versatile Xevo TQ-Absolute that offers superior performance for your quantitative workflows.

  • Targeted Proteomics Applications
  • Targeted Lipidomics Applications
  • Targeted Metabolomics Applications

High performance in compact form

High performance in compact form
Xevo TQ-S micro Triple Quadrupole Mass Spectrometry

Obtain superior performance in routine quantitation, exceptional analytical flexibility, and outstanding sensitivity with the Xevo TQ-S micro that can reproduce high quality analytical performance injection after injection, even with the most complex sample matrices.

  • Targeted Metabolomics Applications
  • Targeted Lipidomics Applications

Complex chromatography challenges, solved

Complex chromatography challenges, solved
ACQUITY Premier System

Enhance the quantitative performance of your LC-MS/MS assay and minimize the impact of non-specific binding with Waters ACQUITY Premier UPLC System.

  • Targeted Proteomics Applications
  • Targeted Metabolomics Applications
  • Targeted Lipidomics Applications

Easy to access sensitivity with the turn of a key

Easy to access sensitivity with the turn of a key
ionKey/MS

Integrate microflow UPLC directly into the source of the mass spectrometer with the ionKey/MS System for an increased level of sensitivity, chromatographic performance, and ease-of-use in targeted proteomics assays.

  • Targeted Proteomics Applications

Let your LC inlet do the work for your nano- to micro-scale separations

Let your LC inlet do the work for your nano- to micro-scale separations
ACQUITY UPLC M-Class System

Achieve the sensitivity of micro- and nanoflow liquid chromatography with UPLC technology with the ACQUITY UPLC M-Class System, accommodating overflow rates from 200 nL/min to 100 µL/min and column inner diameters (I.D.) from 75 µm to 1.0 mm.

  • Targeted Proteomics Applications

Compliance-ready, networkable applications-based software to streamline LC-MS data acquisition, processing, and reporting.

Compliance-ready, networkable applications-based software to streamline LC-MS data acquisition, processing, and reporting.

Delivering market-leading versatility and flexibility for all application areas

Delivering market-leading versatility and flexibility for all application areas
MassLynx Mass Spectrometry Software

Add flexibility in converting sample data into valuable knowledge using Waters MassLynx Mass Spectrometry Software with improved performance while acquiring, analyzing, managing, and sharing mass spectrometry information.

  • Targeted Proteomics Applications
  • Targeted Lipidomics Applications
  • Targeted Metabolomics Applications

Streamline quantitative data acquisition and processing

Streamline quantitative data acquisition and processing
MassLynx Quantitation Applications

Eliminate complex time-consuming manual data review and increase your laboratory productivity by converting data into useful information using Waters MassLynx Quantitation Applications, optimizing your data analysis and processing requirements.

  • Targeted Proteomics Applications
  • Targeted Lipidomics Applications
  • Targeted Metabolomics Applications

Quality consumables to support your targeted omics success.

Quality consumables to support your targeted omics success.

Speed. Sensitivity. Resolution. Confidence. You can have it all.

Speed. Sensitivity. Resolution. Confidence. You can have it all.
ACQUITY Premier Columns

Reduce the risks associated with unpredictable chromatography with ACQUITY Premier Columns with MaxPeak High Performance Surfaces (HPS) Technology, which allows you to see and do more by reducing analyte/surface interactions.

  • Targeted Proteomics Applications
  • Targeted Lipidomics Applications
  • Targeted Metabolomics Applications

Achieve testing efficiencies and accurate results using streamlined Waters services and support.

Achieve testing efficiencies and accurate results using streamlined Waters services and support.

Your success is just a click away

Your success is just a click away
Waters Global Services

Optimize your laboratory's productivity and success with Waters Global Services. Maintain peak system performance, minimize downtime, address application challenges, and support stringent compliance requirements.

  • Targeted Proteomics Applications
  • Targeted Lipidomics Applications
  • Targeted Metabolomics Applications

Make science more accessible with Waters Capital

Make science more accessible with Waters Capital
Payment Solutions

Maximize your lab resources and minimize risk with payment options from Waters Capital, which includes innovative solutions to upgrade aging instruments, customized support, and flexible options to bundle your complete laboratory solution in one easy monthly payment – everything you need to advance your science.

  • Targeted Proteomics Applications
  • Targeted Lipidomics Applications
  • Targeted Metabolomics Applications

The data speaks for itself

The data speaks for itself
Overlay of extracted MRM chromatograms
Overlay of extracted MRM chromatograms for each of the light and heavy stable isotope labeled peptides in the mixture for a 0.5 μL injection in the presence of E. coli background matrix. Red indicates the light and blue the heavy labeled analogue.
Variation in bile acid abundance
Variation in bile acid abundance following dosing with methapyrilene at 50 and 150 mg/kg in Rodent Toxicology Study.
Quantitative measurement consistency
Quantitative measurement consistency for peptide AVQQPDGLAVLGIFLK, where brown indicates the light peptide analogue analyzed by ionKey/MS System.
Negative ion mode chromatogram representing HILIC separation of different lipid classes in plasma
Negative ion mode chromatogram representing HILIC separation of different lipid classes in plasma. The inset chromatogram shows a zoom of the different lipid classes.

Webinars and Resources

  • Application Notebook

Peptide and Protein Bioanalysis

Peptide and Protein Bioanalysis
Download
  • Infographic

Targeted Omics Method Library

Targeted Omics Method Library
Download

Related

Proteomics
Waters proteomics solutions and expertise are leading resources for proteomics discovery research. Our MS-based systems maximize efficiency and provide unique tools for protein discovery, characterization, quantification, and validation.

Lipidomics
Combine deep coverage of cellular lipids and the robustness and precision for large studies with Waters lipidomics solutions.

Metabolomics
Enhance your workflows for discovery and targeted metabolomics with Waters solutions.

Learn more about Targeted Omics and Biomarker Quantification Solutions.

Learn more about Targeted Omics and Biomarker Quantification Solutions.
Protein used as biomarker of kidney function
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Targeted omics and biomarker quantification is the process of measuring specific molecules in biological systems to understand their role in health, disease, and treatment response. Unlike broad discovery studies that analyze thousands of molecules at once, targeted omics focuses only on a selected set of known or suspected molecules such as proteins, lipids, or metabolites that are linked to a particular biological process or condition. This focused approach provides greater accuracy and consistency, making it highly valuable for research and clinical applications.

The main advantage of targeted omics is its ability to measure biomarkers with very high sensitivity and precision. Biomarkers are molecules that can show whether the body is functioning normally, if a disease is developing, or how well a treatment is working. Advanced technologies like liquid chromatography coupled with mass spectrometry (LC-MS) make this possible by accurately detecting and quantifying these molecules even when they are present in very small amounts. Combining LC and MS helps separate and identify compounds in complex samples with confidence.

Targeted omics is widely used in different areas of science and healthcare. In clinical research, it helps confirm potential biomarkers discovered in earlier studies before they are used in diagnostics or patient care. In drug development, it supports studies on how a drug behaves in the body, how effective it is, and whether it reaches its intended target. It is also important for personalized medicine, where biomarkers guide doctors in choosing the most effective treatment for each patient.

For the best results, targeted omics requires advanced instruments and well-optimized workflows. Automated sample preparation, high-quality LC-MS systems, and powerful data analysis tools ensure accurate and reproducible results that meet regulatory standards. By enabling precise measurement of important molecules, targeted omics serves as a key link between early research and real-world clinical applications, helping to turn scientific discoveries into better healthcare solutions.