• Application Note

Camphorsulfonic Acid in Rat Plasma on Oasis WAX

Camphorsulfonic Acid in Rat Plasma on Oasis WAX
  • Waters Corporation
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Animal experiments for urine collection using white rats

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of camphorsulfonic acid in rat plasma on Oasis Wax.

Introduction

Camphorsulfonic acid is a strong acid with a pKa of approximately 1.5. The best SPE recoveries for this type of acid are on Oasis WAX products.

Experimental

Test Conditions

Oasis WAX 10 mg 96-Well Plate

Condition:

500 μL MeOH

Equilibrate:

500 μL H2O

Load:

500 μL (250 μL rat plasma, diluted 1:1 with 4% H3PO4)

Wash 1:

500 μL 2% FA, pH 2.7

Wash 2:

500 μL MeOH

Elute:

250 μL (125 μL x 2) 5% NH4OH in MeOH

Options:

1. Dilute 250 μL H2O with 2% FA

2. Evaporate/ Reconstitute

3. Direct inject

Inject:

10 μL

Oasis WAX 96-Well Plate μ Elution Plate

Condition:

200 μL MeOH

Equilibrate:

200 μL H2O

Load:

100 μL (50 μL rat plasma diluted 1:1 with 4% H3PO4)

Wash 1:

200 μL 2% FA, pH 2.7

Wash 2:

200 μL MeOH

Elute:

50 μL (25 μL x 2) 5% NH4OH in MeOH

Options:

1. Direct injection

2. Dilute with 50 μL H2O with 2% FA

3. Evaporate/ Reconstitute

Inject:

10 μL

Column:

SunFire C18 2.1 x 20 mm IS, 3.5 μm

Mobile phase A:

10 mM CH3COO-NH4+, pH 5.5

Mobile phase B:

MeOH with 10 mM CH3COO-NH4+, pH 5.5

Flow rate:

0.4 mL /min

Injection volume:

10 μL

Column temp:

Ambient

Instrument:

2777 Sample Manager, 1525μ Binary HPLC Pump and Quattro Premier

Quattro Premier

ESI- source temp:

150 °C

Desolvation temp:

350 °C

Cone gas flow:

50 L /Hr

Desolvation gas flow:

600 L /Hr

Collision cell:

2.2e-3 bar (Ar gas)

Results and Discussion

WA60084, June 2007

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