This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of nadolol.
The compound analyzed in this study was nadolol using Symmetry Shield RP18 Column.
USP Tailing Factors
            
        | 
                     Column:  | 
                     Symmetry Shield RP18, 4.6 x 250 mm, 5 μm  | 
            
| 
                     Part number:  | 
                     186000112  | 
            
| 
                     Mobile phase:  | 
                     Methanol/water 35:65, 2.93 g of sodium chloride, 1.0 mL of 0.1 N hydrochloric acid  | 
            
| 
                     Flow rate:  | 
                     1.0 mL/min  | 
            
| 
                     Injection volume:  | 
                     20 μL  | 
            
| 
                     Detection:  | 
                     UV @ 220 nm  | 
            
            
        WA31763.105, June 2003