• Application Note

Pharmaceuticals and Personal Care Products in Water, Soil, Sediment and Biosolids by HPLC-MS/MS

Pharmaceuticals and Personal Care Products in Water, Soil, Sediment and Biosolids by HPLC-MS/MS
  • Waters Corporation
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Abstract

In this Application, we monitor the presence of pharmaceuticals in drinking water supplies and examining their long term effects on human health.

Introduction

Many hundreds of active compounds are used in both human and veterinary drug formulations. Due to the many different applications related to pharmaceuticals, their residues can reach the environment in multiple ways including excretion and manufacturing discharge. These compounds are not completely eliminated via sewage treatment plants, thus they can reach surface and groundwater supplies. Recently, there has been increased interest in monitoring for the presence of pharmaceuticals in drinking water supplies and examining their long term effects on human health.

Experimental

HPLC conditions

Instrument:

Waters 2690 HPLC or Waters 2795 HPLC, Quattro Ultima MS/MS

LC Column:

Waters XTerra C18, 3.5 μm, 10.0 cm, 2.1 mm

Ionizatoin:

Electrospray Positive (ES+)

Acquisition:

MRM mode, unit resolution

Injection Volume:

15 μL

1 Solvent A = 0.3% Formic Acid and 0.1% Ammonium Formate in HPLC water
  Solvent B = 1:1 Acetonitrile:Methanol

Group 1 – Acidic extraction, positive electrospray ionization (ESI+) instrument conditions.

Continuation of above table

Group 1 acidic extraction, positive electrospray ionization (ESI+) compound retention times (RTs), parent-daughter transitions, quantitation references, method detection limits, and minimum levels of quantitation.

HPLC conditions

Instrument:

Waters 2690 HPLC or Waters 2795 HPLC, Quattro Ultima MS/MS

LC Column:

Waters XTerra C18, 3.5 μm, 10.0 cm, 2.1 mm

Ionization:

Negative Ion Electrospray

Acquisition:

MRM mode, unit resolution

Injection Volume:

5 μL

1 Solvent A = 1:1 acetonitrile:methanol, with 5 mM Oxalic Acid
Solvent B = HPLC H2O, with 5 mM Oxalic Acid.

Group 2 – Acidic extraction positive electrospray ionization (ESI+) instrument conditions.

1 Isochlortetracycline (ICTC) is reported as the sum ICTC + ECTC due to a common transition ion.

Group 2 acidic extraction positive electrospray ionization (ESI+) compound retention times (RTs), parent-daughter transitions, quantitation references, method detection limits, and minimum levels of quantitation.

HPLC conditions

Instrument:

Waters 2690 HPLC or Waters 2795 HPLC, Quattro Ultima MS/MS

LC Column:

Waters XTerra C18, 3.5 μm, 10.0 cm, 2.1 mm

Ionization:

Negative Ion Electrospray

Acquisition:

MRM mode, unit resolution

Injection Volume:

5 μL

1 Solvent A = 0.1% Ammonium Acetate and 0.1% Acetic Acid in HPLC water
Solvent B = 1:1 MethanolAcetonitrile

Group 3 Acidic extraction negative electrospray ionization (ESI-) instrument conditions.
Group 3 acidic extraction negative electrospray ionization (ESI-) compound retention times (RTs), parent-daughter transitions, quantitation references, method detection limits, and minimum levels of quantitation.

HPLC conditions

Instrument:

Waters 2690 HPLC or Waters 2795 HPLC, Quattro Ultima MS/MS

LC Column:

Waters Atlantis HILIC, 3.0 μm, 2.1x 100 mm

Ionization:

Electrospray Positive (ES+)

Acquisition:

MRM mode, unit resolution

Purge Solvent:

100% CH3CN (changed from H2O)

Injection Volume:

2.0 μL

1 Solvent A = 0.1% Acetic Acid/Ammonium Acetate Buffer
Solvent B = Acetonitrile

Group 4 – Basic extraction positive electrospray ionization (ESI+) instrument conditions.
Group 4 basic extraction positive electrospray ionization (ESI+) compound retention times (RTs), parent-daughter transitions, quantitation references, method detection limits, and minimum levels of quantitation.

References

  1. Total Solutions for Environmental Applications: 720002163EN
  2. LC-MS Determination of Pharmaceutical Residues in Environmental Samples: 720000421EN

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720002734, August 2008

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