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Ibuprofen and Naproxen in Serum

Ibuprofen and Naproxen in Serum
  • Waters Corporation
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Technician holding test tube examining human centrifuged serum

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of ibuprofen and naproxen in serum.

Introduction

The compounds used in this study are –

  1. Naproxen
  2. Ibuprofen

Experimental

HPLC Method

Column:

Symmetry Shield RP8, 3.0 x 150 mm, 5 μm

Guard column:

Sentry Guard Column, 3.9 x 20 mm, 5 μm

Part numbers:

Column - WAT094243, Guard - WAT200675

Mobile phase:

1.2% glacial acetic acid in water, Acetonitrile 50:50

Flow rate:

0.6 mL/min

Injection:

20 μL of reconstituted porcine serum extract

Temperature:

25 °C

Detection:

UV @ 254 nm

Results and Discussion

Featured Products

WA31763.90, June 2003

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