• Nota applicativa

Verapamil in Plasma - LC/UV

Verapamil in Plasma - LC/UV
  • Waters Corporation
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Verapamil molecular structure

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of verapamil in plasma using XTerra Phenyl columns 

Introduction

Compounds used in this application brief are:

1. Verapamil

2. Methoxyverapamil (ISTD)

3. Norverapamil

Experimental

HPLC Method

Column:

XTerra Phenyl, 4.6 x 100 mm, 3.5 μm (p/n: 186001139)

Mobile phase A:

H2O

Mobile phase B:

ACN

Mobile phase C:

100 mM NH4HCO3 in H2O, pH 9.0

Flow rate:

1.4 mL/min

Isocratic mobile phase composition:

53% A; 37% B; 10% C

Injection volume:

20 μL

Temperature:

30 °C

Detection:

UV @ 230 nm

Instrument:

Alliance 2695, 2996 PDA

Results and Discussion

Featured Products

WA20738.114, June 2002

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