XBridge Protein BEH SEC Column, 200Å, 2.5 µm, 4.6 mm X 150 mm, 1/pk

SKU: 186009175
XBridge Protein BEH SEC Column, 200 Å, 2.5 µm, 4.6 mm X 150 mm, 1/pk

XBridge Protein BEH SEC Column | 186009175

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Product Description

XBridge Protein BEH SEC Column, 200 Å, 2.5 µm, 4.6 mm X 150 mm Columns, containing 2.5 µm particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns. They are best suited to separate proteins ranging from 10,000 to 450,000 Daltons. Each batch is specifically QC tested using Waters BEH200 SEC Protein Standard (p/n: 186006518) to help ensure consistent performance for challenging size-based protein separations.

Note: This item is also available with one vial the BEH200 SEC Protein Standard at no additional cost (p/n: 176004335).

Suggested Use: Gel Filtration, Size Exclusion Chromatography, Aggregate, Monomer and Fragment analysis

If you are developing a new method, please consider our newer technology XBridge Premier Protein 250Å SEC and ACQUITY Premier Protein 250Å SEC Columns for superior recovery, separation, and resolution.

Specifications

  • Chemistry

    SEC

  • Separation Mode

    Gel Filtration (aqueous)

  • Particle Substrate

    Hybrid

  • pH Range Min

    2.5 pH

  • pH Range Max

    8 pH

  • Temperature Limits

    60 C

  • Maximum Pressure

    4500 psi (310 Bar)

  • Endcapped

    No

  • Bonding Technology

    Diol (OH)

  • Silanol Activity

    Low

  • Molecular Weight Range Min

    10000

  • Molecular Weight Range Max

    450000

  • Particle Size

    2.5 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    200 Å

  • QC Tested

    Protein

  • Format

    Online Column

  • Packing Solvent

    20% methanol in water

  • Surface Area

    220

  • System

    HPLC, UHPLC, UPLC

  • Particle Technology

    BEH

  • Technique

    LC

  • USP Classification

    L20, L33, L59

  • Inner Diameter

    4.6 mm

  • Length

    150 mm

  • Carbon Load

    12 %

  • eCord

    No

  • UNSPSC

    41115711

  • Application

    Protein

  • Brand

    XBridge

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents

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XBridge Protein BEH SEC Column, 200 Å, 2.5 µm, 4.6 mm X 150 mm, 1/pk

Invest in the XBridge Protein BEH SEC Column so you can perform analyses and make sure your analysis is producing the best results.

The Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating utilized in our popular range of HPLC-based SEC columns provide the foundation for the 2.5 µm particle size, 200 Å pore size XBridge Protein BEH SEC columns. These laboratory tools work best when separating proteins with a size between 10,000 and 450,000 Daltons.

You can benefit from the easy migration approach to UltraPerformance LC technology by utilizing the XBridge Protein BEH SEC Column. Due to its unrivaled column longevity and versatility to operate over an unmatched range of mobile phase pH and temperatures, the column is a crucial piece of lab equipment.

The XBridge Protein BEH SEC Column uses packing materials that are produced in a cGMP, ISO 9001-certified facility using ultra-pure reagents and were created expressly for use with the ACQUITY UPLC System. To ensure repeatable performance, each batch of XBridge Protein BEH SEC Column packing material is chromatographically tested with acidic, basic, and neutral analytes, and the results are held to limited specification ranges. On the eCordTM Intelligent Chip, a Performance Chromatogram and Certificate of Batch Analysis are supplied after each column has undergone an individual test.

Use the XBridge Protein BEH SEC Guard Column, 200Å, 2.5 µm, 4.6 mm X 30 mm, 1/pk in conjunction with the XBridge Protein column in order to prevent any chemical or particulate contaminants from entering the mobile phase and interfering with the analysis. The guard column is made using the same packing materials that are present in the column, making the two lab equipment well suited to work together. It also helps in delivering more accurate results and ensures that the column lasts a long time.

Is There A Minimum And Maximum pH Limit For The XBridge Protein BEH SEC Column?

The XBridge Protein BEH SEC Column listed here can work well within the 2.5-8 pH range.

What Are The Temperature Limits Of The XBridge Protein BEH SEC Column?

The Waters XBridge Protein BEH SEC Column listed here is able to work with a maximum of 60 C.