• Application Note

Analysis of Ginseng Root Powder on an XBridge HPLC Amide Column

Analysis of Ginseng Root Powder on an XBridge HPLC Amide Column

  • Waters Corporation

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of ginseng root powder on an XBridge HPLC Amide column.

Introduction

Compound

Experimental

HPLC Conditions

Column:

XBridge Amide, 3.5 μm, 4.6 x 150 mm

Part Number:

186004869

Mobile Phase:

80:20 MeCN:H2O

Isocratic Flow Rate:

1.4 mL/min

Column Temp.:

60 °C

Sample Temp.:

10 °C

Injection Vol.:

11.5 μL

Needle Wash:

95:5 MeCN:H2O

Seal Wash:

10:90 MeOH:H2O

UV:

203 nm

Sampling Rate:

20 Hz

Filter Time Constant:

0.1 sec

Total Run Time:

8 min

Instrument:

Alliance 2695 and 2998 PDA

Extraction Procedure

  1. Centrifuge for 5 min @ 10,000 rpm.
  2. Keep supernatant and re-extract by repeating steps 2-4 two more times.
  3. Pool all three extracts and mix well.
  4. Filter through a 13 mm nylon 0.2 μm filter prior to injection.

Results and Discussion

WA64082, February 2010

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