This is an Application Brief and does not contain a detailed Experimental section.
This application brief details on analysis of cyclizine and chlorcyclizine using Symmetry Columns.
The compounds analyzed in this study are:
            
        | 
                     Column:  | 
                     Symmetry C8, 3.9 x 150 mm, 5 µm  | 
            
| 
                     Guard column:  | 
                     Symmetry Guard Column 3.9 x 20 mm, 5 µm  | 
            
| 
                     Part numbers:  | 
                     Column - WAT046970, Guard - WAT054250  | 
            
| 
                     Mobile phase:  | 
                     7.4 mM sodium 1-heptanesulfonate in water/acetonitrile 65:35  | 
            
| 
                     Flow rate:  | 
                     1.0 mL/min  | 
            
| 
                     Injection volume:  | 
                     8 µL of 100 µg/mL sample  | 
            
| 
                     Detection:  | 
                     UV @ 230 nm  | 
            
            
        WA31763.65, June 2003