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Chlorpromazine in Rat Plasma

Chlorpromazine in Rat Plasma
  • Waters Corporation
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Platelet-rich plasma vials in rack

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of chlorpromazine using XTerra MS C18 columns.

Introduction

Chlorpromazine in rat plasma has been analyzed in this application brief.

Experimental

HPLC Conditions

Column:

XTerra MS C18 2.1 x 30 mm, 3.5 μm (p/n: 186000398)

Mobile phase A:

0.2% NH4OH

Mobile phase B:

ACN

Flow rate:

0.2 mL/min

Isocratic mobile phase composition:

40% A; 60% B

Injection volume:

20 μL

Detection:

MS ESI-

Instrument:

Alliance 2790, Micromass Quattro Ultima

MS Conditions

Ion source:

ESI+

Source temp.:

150 °C

Gas cell:

1.5e-3 mbar, 20 eV

Desolvation temp.:

350 °C

Cone gas flow:

150 L/hr

Drying ga flow:

600 L/hr

Cone voltage:

40 V

Results and Discussion

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WA20738.024, June 2002

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