ACQUITY Premier Protein SEC Column, 250 Å, 1.7 µm, 4.6 x 300 mm Method Validation Kit Columns help scientists obtain reliable protein size variant separations (10,000 to approx 650,000 Daltons) via the synergistic coupling of Waters MaxPeak Premier High Performance Surfaces and novel BEH-PEO Particle technologies. These advancements in SEC column hardware and SEC particle technology work to minimize non-desired secondary ionic or hydrophobic interactions between proteins and the column to minimize costly method development work by allowing chromatographers to use a "generic" or "platform-type" method for many samples. Each batch is specifically QC tested using Waters mAb Size Variant Standard (p/n: 186009429) to help ensure consistent performance for challenging size-based protein separations.
Note: This Method Validation Kit (MVK) contains (3) (p/n: 186009959) columns from (3) different batches.
Chemistry |
SEC |
Separation Mode |
SEC |
Particle Substrate |
Hybrid |
pH Range Min |
2.5 pH |
pH Range Max |
8 pH |
Temperature Limits |
60 C |
Maximum Pressure |
11000 PSI (759 bar) |
Endcapped |
No |
Bonding Technology |
BEH-PEO |
Silanol Activity |
Low |
Molecular Weight Range Min |
10000 |
Molecular Weight Range Max |
650,000 |
Particle Size |
1.7 µm |
Endfitting Type |
Parker-style |
Pore Size |
250 Å |
QC Tested |
Protein |
Format |
Premier Column |
Surface Area |
174 |
System |
UHPLC, UPLC |
Particle Technology |
BEH |
Technique |
LC |
USP Classification |
L33, L59 |
Inner Diameter |
4.6 mm |
Length |
300 mm |
eCord |
Yes |
UNSPSC |
41115709 |
Application |
Protein |
Brand |
ACQUITY |
Product Type |
Columns |
Units per Package |
3 pk |