Biphenyl Columns with MaxPeak Premier Technology
Alternative selectivity. Performance without compromise.
Method development often requires more than hydrophobic retention. When C18 or phenyl stationary phases cannot fully resolve compounds of interest, chromatographers need an alternative selectivity tool that integrates seamlessly into existing reversed-phase workflows.
Push beyond the limits when traditional phases fall short with Waters Biphenyl Columns. Engineered to deliver enhanced aromatic selectivity, Waters Acquity Biphenyl Columns and XBridge Biphenyl Columns offer 10× lower UV bleed than competitive biphenyl columns and maintain true pH stability from 1.5 – 10. Built on proven Ethylene Bridged Hybrid (BEH) particle technology and packed in MaxPeak High Performance Surfaces (HPS) Technology-based hardware, they provide the reproducibility, robustness, and sensitivity modern LC and LC-MS workflows demand.
Biphenyl Columns with MaxPeak Premier Technology
Specifications
Overview
- Enhance π–π interactions alongside hydrophobic and steric contributions for differentiated selectivity even with challenging mixtures
- Support stable performance across a broad pH range with proven BEH particle technology with trifunctional bonding
- Adjust pH as a selectivity lever without compromising UV compatibility or robustness
- Improve retention of compounds containing π electrons
- Expand selectivity beyond traditional C18 and phenyl stationary phases
- Develop robust methods with stable performance across common pH conditions
Recommended Use: Alternative selectivity in method development for small molecule pharma, bioanalytical, and food and environmental applications.
Optimized for UV detection
Many biphenyl stationary phases can introduce column bleed, interfering with low-level quantitation and baseline stability. Waters Biphenyl Columns demonstrate more than 10× lower UV-detected column-bleed than competitive biphenyl columns under acidic gradient conditions. This performance results in stable baselines and more reliable peak integration, enabling greater confidence when using UV detection in both method development and QC environments.
With Waters, your lab can achieve:
- Flatter baselines under low pH gradient conditions
- More accurate peak integration at low levels
- Improved sensitivity and quantitative confidence
Stable performance from pH 1.5–10
Chromatographers rely on mobile phase pH as a primary selectivity lever. Waters Biphenyl Columns are engineered with proprietary trifunctional bonding on BEH particle technology, delivering robust stability across a wide pH range. This allows you to explore acidic and basic method conditions with confidence and durability.
With Waters Biphenyl Columns, you can ensure:
- A true operating range from pH 1.5 to 10
- Stability under both acidic and basic conditions
- Long-term performance without premature bonded phase loss
Batch-to-batch reproducibility and robustness
Consistent selectivity is critical for successful method transfer and regulated workflows. Built on the proven BEH particle platform and engineered with controlled bonding chemistry, Waters Biphenyl Columns deliver industry-leading batch-to-batch reproducibility. Performance has been validated across multiple batches and particle sizes to ensure consistent retention, selectivity, and peak shape.
With Waters, your lab can experience:
- Consistent retention, selectivity, and peak shape across batches
- Reproducibility and robustness across particle sizes
- Confidence for validated and regulated methods
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