• Applikationsbericht

Cytochrome c Tryptic Digest - Pore Size Effects

Cytochrome c Tryptic Digest - Pore Size Effects
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief details on cytochrome c tryptic digest pore size effects using Symmetry Columns.

Introduction

The compound used in this study is cytochrome c tryptic digest.

Experimental

HPLC Method

Column:

Symmetry C18, 4.6 x 150 mm, 5 µm, 100Å

Guard column:

Symmetry 300 C18, 4.6 x 150 mm, 5 µm, 300Å

Part numbers:

Column - WAT045905, Guard - WAT106157

Mobile phase A:

0.1% TFA in Water

Mobile phase B:

0.1% TFA in Acetonitrile

Flow rate:

0.75 mL/min

Injection volume:

20 µL

Temperature:

35 °C

Detection:

UV @ 214 nm

Gradient

Gradient

Results and Discussion

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WA31763.67, June 2003

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