HPLC familiarity with UPLC performance
If you are performing routine analyses or developing methods, or just prefer the flexibility of multi-solvent capabilities in a quaternary-based system, the only choice has been HPLC. Until now.
The ACQUITY UPLC H-Class is a streamlined system that brings together the flexibility and simplicity of quaternary solvent blending and a flow-through-needle injector to deliver the advanced performance expected of UPLC type separations – high resolution, sensitivity and improved throughput – while maintaining the robustness and reliability that ACQUITY systems are known for.
Choosing the ACQUITY UPLC H-Class enables you to continue running existing HPLC methods on a forward-looking LC platform that allows you to confidently and seamlessly transition to UPLC separations, when you’re ready, using integrated system tools and reliable column kits for method transfer and method development that simplify migration.
With the ACQUITY UPLC H-Class, your laboratory can achieve UPLC-quality separations without changing the way you work. The familiar design of the ACQUITY UPLC H-Class’s Quaternary Solvent Manager (QSM) and Sample Manager (SM-FTN), with flow-through needle design, gives you all the flexibility and usability of your current HPLC while still achieving the highly efficient separations that only UPLC can provide.
With ACQUITY UPLC H-Class, you won't have to think twice about transferring methods to and from any LC instrument.
With unprecedented selectivity and reproducibility across batches and particle sizes, our Method Transfer Kits preserve the integrity of a separation as it is transferred to and from HPLC and UPLC platforms. And choosing the right UPLC columns and conditions couldn't be easier with the method transfer guidelines provided by the ACQUITY UPLC Columns Calculator, available on the system console.
In this method transfer example using the related substances test for galantamine, used in the treatment of Alzheimer's disease, the USP method (monograph: USP32-NF27 Supplement: No. 2, Page 4245) is demonstrated first using an HPLC system. The method is directly transferred to the ACQUITY UPLC H-Class System using an HPLC column, maintaining selectivity and resolution, then scaled to UPLC using the Columns Calculator and optimized for the shortest analysis time at equal peak capacity. The run time decreased by 46 minutes.
See how the ACQUITY UPLC H-Class System's Quaternary Solvent Manager and Sample Manager operate.
Turn a tedious and time-consuming process with HPLC into a versatile and productive workflow with UPLC.
ACQUITY UPLC H-Class features a variety of components to improve data quality, increase sample throughput, and reduce cost per analysis for timely and predictable results.
ACQUITY UPLC Method Development Kits offer combinations of different column chemistries to accommodate your method development approach, enabling methods to be developed efficiently and effectively.
Separation of clozapine from four structurally-related impurities using BEH Column Technology. These columns can be quickly screened over a wide range of temperature, mobile phase pH, and pressure to maximize selectivity and produce a successful result
Simultaneous method development for morphine-related compounds using reversed-phase and HILIC chromatography. New system capabilities inherent to the ACQUITY UPLC H-Class enable the sequential development of methods for compounds with a broad range of polarity and chemical properties. Compounds: (1) 6-acetylmorphine, (2) morphine, (3) morphine-3β-D-glucuronide.
The ACQUITY UPLC H-Class features a quaternary gradient pumping system that can continuously blend four solvents for use in generating a chromatographic separation. Auto•Blend technology uses this capability to automate the formulation of mobile phases from reservoirs of pure solvents or concentrated stock solutions.
Auto•Blend technology can be applied to any combination of solvents, modifiers, buffers, and salts. Any sequence of isocratic, binary, ternary, or quaternary gradients can be utilized. We can then draw any percentage we want from any of those bottles to adjust the separation or actually create on-demand the mobile phase that we require for the separation.
Since mobile phase combinations and gradients are automatically blended on-demand to meet the optimum retention and selectivity of the separation, this technology is useful for routine assays, in complicated separations requiring multiple solvents, and in automatic method development or system flushing.
Auto•Blend Plus technology further extends this capability by automatically managing pH and ionic strength requirements for the mobile phase. The software calculates the proportions of buffer stocks required for desired conditions. Computation can be based on known pK values or on an empirical calibration table, making any possible buffer combination available. Users can also create their own library of combinations.
For example, in the separation of a mixture of analgesics, the ACQUITY UPLC H-Class has proven very suitable in providing a high-quality chromatogram. What we've also found is that we can use this quaternary system as a way to make our work easier and also to eliminate some of the sources of error.
In a binary separation, we run a gradient from a high percentage of water to a high percentage of organic solvent. We manually prepare an A solvent (water and formic acid) and a B solvent (acetonitrile and formic acid). We draw a portion of the flow from each of those two bottles and change that proportion as a function of time during the separation. We get very reproducible chromatography. But that chromatography depends on how well we mixed the solvents in those two bottles.
Alternatively, we can use the ACQUITY UPLC H-Class System in Auto•Blend mode. Here, instead of making two bottles of pre-formulated solvent, we use the A and the B solvent as pure water and pure acetonitrile. In the D line, we put a ten-fold concentrated solution of formic acid in water. We then draw from three of the four bottles to create the same chromatographic method.
When we take 88% of the flow from the A bottle and 10% of the flow from the D bottle, we still have the same percentage of formic acid in water at our initial conditions. As we change the proportions of water and acetonitrile during the gradient, the constant percentage flow from the D line ensures the required pH and salt concentration.
When we compare the ternary gradient with the binary gradient, we observe the same chromatography, exactly the same analytical results. So the ternary gradient does not compromise the quality of our analyses and gives us the opportunity to avoid errors and to put less effort into doing this routine work.
The advantage is that instead of having to measure out two pre-mixed solvent bottles, we only have to make the one measurement for the concentrated stock. It's easier to make one bottle than two, and because we're making fewer measurements, we have fewer opportunities for an error to creep into our measurements.
Auto•Blend and Auto●Blend Plus technologies make routine analyses easier to manage, easier to execute by reducing the amount of work to be done in preparing complex mobile phases, easier to transfer methods to other laboratories, and it makes the whole laboratory process more efficient by reducing the possibility for errors during the separation.
Get mass spectral data, and ensure the most complete separation for your methods.
Within the same analysis. Within the same workflow.
Laboratories around the world are converting to ACQUITY UPLC H-Class.
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Learn how to automate buffer combinations.