Methotrexate analysis with quantitative UPLC-MS/MS for clinical research

Importance of selective detection

Traditional enzyme and fluorescence based immunoassays overestimate the concentration of methotrexate due to cross reactivity of the inactive metabolite 2,4-Diamino-N10methylpteroic acid (DAMPA). This cross reactivity emphasizes the importance of selective detection, which can be achieved with LC-MS/MS using the ACQUITY UPLC I-Class PLUS and Xevo TQD Mass Spectrometer in clinical research laboratories.


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