GlycoWorks 2-AB N-Glycan Kits

Optimized Reductive Amination Protocols

The GlycoWorks Sample Preparation consumable line along with the ACQUITY UPLC BEH Glycan Separation Technology Column is optimized for use with ACQUITY UPLC systems with fluorescence detection (FLR) for separating the released glycans of biopharmaceuticals as their 2-aminobenzamide (2-AB) derivatives.

The GlycoWorks Sample Preparation Consumable Line includes the following features:

Specifically for 2-AB labeled N-Glycans but the protocol can be adapted for 2-AA analysis
Works really well for the market of biopharmaceuticals that includes IgG’s (but not limited to this-just validated with IgG)

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The HILIC Advantage

Hydrophilic Interaction Chromatography (HILIC) can be used to successfully improve retention of very polar species. This technique is rapidly growing for this application area. One reason for this is that this mode of separation is well suited for glycans due to their highly polar nature. The GlycoWorks HILIC SPE provides an efficient and reproducible mechanism for effective glycan cleanup and provides quantitative and consistent recoveries for a diverse range of N-Glycans.

Inclusive Methodology

The GlycoWorks Kit offers a protocol for complete sample processing of released N-glycans with either 2-AB or 2-AA labeling. Two formats offered, one for high-throughput which is a 96-well plate and the other for low volume users in a 1 cc cartridge format.

Kit components include:

Sample cleanup devices with HILIC (amino-propyle) sorbent
- High-throughput: GlycoWorks HILIC µElution SPE Plate (96-well)
- Single Use: GlycoWorks HILIC 1 cc (20 pack) and GlycoWorks HILIC 1 cc Flangeless Cartridges for the positive pressure manifold (20 pack)
GlycoWorks Control Standard (un-labeled Human IgG) for method development/troubleshooting
GlycoWorks Reagent Kit with material for protein deglycosylation and labeling

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Superior Product Support

There is no “one size fits all” solution for released glycan analysis. Due to the complicated nature of the sample preparation process compounded by the complexity of customer samples, product support is critical. The GlycoWorks protocol consists of the following to help aid in customer questions and ease of use when following the protocol:

Care and use with protocols specific for 96-well plate and 1 cc cartridges
Tips and tricks to help guide and support the customer
Application Notes and Tech Briefs
Customer support video

Solutions Benefit

Only one company can provide you with everything you need for released glycan analysis. The GlycoWorks Sample Preparation consumable line was developed and designed to complement the existing Waters Glycan Application Solution on 2-AB labeled N-Glycan, this sample preparation offering and complimentary standards provides a unique, comprehensive, yet versatile, solution.

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