Literature


Description
Retention Time, Product Ion, and CCS Characterization of the Constituents of a FDA-Approved Small Mo...
Library Number: APNT135082547 | Format: PDF | File Size: 760.42 kB | Year: 2021 | Content Type: Application Notes

We have generated an extensive library of FDA approved drug small molecules and used it to perform a non-targeted urinary screen of a patient sample to identify administered pharmaceutical compounds.

Enhancing Phosphopeptide Quantitation using PREMIER Peptide CSH C18 Columns
Library Number: APNT135082396 | Format: PDF | File Size: 430.99 kB | Year: 2021 | Content Type: Application Notes

In this application note, we investigate the recovery and quantitative performance of phosphorylated and non-phosphorylated peptide analysis using PREMIER Peptide CSH C18 columns, which feature MaxPeak High Performance Surface (HPS) Technology.

Plasmid Isoform Separation and Quantification by Anion-Exchange Chromatography (AEX)
Library Number: APNT135082325 | Format: PDF | File Size: 606.83 kB | Year: 2021 | Content Type: Application Notes

This application note shows various levels of the three major isoforms of the plasmid DNA, namely, supercoiled, open circular, and linear form, can be separated and quantified on a Waters Protein-Pak Hi Res Q Column fitted to an ACQUITY UPLC H-Class Bio S...

A Comparison of PREMIER Columns with MaxPeak HPS Technology versus PEEK-lined Column Hardware
Library Number: APNT135082328 | Format: PDF | File Size: 546.5 kB | Year: 2021 | Content Type: Application Notes

In this application brief, we have compared MaxPeak HPS column hardware to PEEK-lined stainless-steel hardware from two different vendors.

Increasing Recovery and Chromatographic Performance of “Acidic” Peptides Using Waters ACQUITY PREMIE...
Library Number: APNT135082322 | Format: PDF | File Size: 419.49 kB | Year: 2021 | Content Type: Application Notes

This application brief demonstrates how the chromatographic performance gains observed with ACQUITY PREMIER featuring MaxPeak HPS technology can increase productivity in the lab and improve data quality for metal-sensitive analytes.

Analysis of Cannabinoids in Cannabis Plant Materials and Edible Products Using UltraPerformance Liqu...
Library Number: APNT135082330 | Format: PDF | File Size: 1919 kB | Year: 2021 | Content Type: Application Notes

In this study, an ACQUITY UPLC H-Class System with both the ACQUITY PDA and the ACQUITY QDa Mass Detector was used for the analysis of 18 cannabinoids in cannabis flower, hemp, and edible products, including gummies, chocolates, and beverages.

Development of a Robust Method for Analysis of Aspirin and Related Substances Using a Statistical So...
Library Number: APNT135082347 | Format: PDF | File Size: 1595.7 kB | Year: 2021 | Content Type: Application Notes

In this application note, we develop a MS compatible method for aspirin API and its related substances using a Fusion QbD method development software.

Improved Peak Recovery and Peak Shape of Oligonucleotides Using Waters PREMIER Columns
Library Number: APNT135082201 | Format: PDF | File Size: 201.63 kB | Year: 2021 | Content Type: Application Notes

This application brief demonstrates to drastically reduce the undesirable interaction of oligonucleotides with column hardware using a standard LC instrument and two separate columns.

Determination of Cationic Polar Pesticides and Plant Growth Regulators Using UPLC-MS/MS with the ACQ...
Library Number: APNT135082210 | Format: PDF | File Size: 1163.95 kB | Year: 2021 | Content Type: Application Notes

This application note demonstrates a single extract LC-MS/MS method for the determination of highly polar cationic pesticide residues and plant growth regulators in several food commodities that meet or exceed the MRL detection requirements in the Europea...

Comprehending COVID-19: Mixed-Mode Chromatography for Ion Pairing Free LC-MS of Remdesivir and Remde...
Library Number: APNT135082213 | Format: PDF | File Size: 459.77 kB | Year: 2021 | Content Type: Application Notes

In this application note, we take advantage of hybrid silica surfaces and mixed mode chromatography with ammonium acetate mobile phase to devise a starting point for a method that could potentially be applied to in vitro cell assays or pharmacokinetic ana...

Automated Preparation and LC-MS/MS Analysis of Methylmalonic Acid in Serum Using the Andrew+ Pipetti...
Library Number: APNT135082229 | Format: PDF | File Size: 451.97 kB | Year: 2021 | Content Type: Application Notes

This application brief demonstrates the automated extraction of MMA from serum employing the Ostro Plate for protein precipitation and phospholipid removal on the Andrew+.

Ginseng Compound Screening using the ACQUITY RDa Detector
Library Number: APNT135082217 | Format: PDF | File Size: 552.19 kB | Year: 2021 | Content Type: Application Notes

This application note demonstrates the rapid, accurate screening of ginseng utilizing the Waters ACQUITY RDa Detector, a high-resolution LC-MS time-of-flight (ToF) mass analyser.

Automated High Throughput Sample Preparation and UPLC-MS/MS Analysis for the Quantification of EtG a...
Library Number: APNT135082016 | Format: PDF | File Size: 592.24 kB | Year: 2021 | Content Type: Application Notes

This work demonstrates an automated simple dilute and shoot sample preparation method using the Andrew+ Pipetting Robot from Andrew Alliance.

ACQUITY UPLC I-Class/Xevo TQ-S micro IVD System: Analytical Performance for Phylloquinone
Library Number: APNT135082018 | Format: PDF | File Size: 256.12 kB | Year: 2021 | Content Type: Application Notes

This document describes a test of the analytical performance of the ACQUITY UPLC I-Class/Xevo TQ-S micro IVD System for the analysis of vitamin K1 in serum.

RADIAN ASAP with LiveID – Fast, Specific, and Easy Drug Screening
Library Number: APNT135082020 | Format: PDF | File Size: 877.43 kB | Year: 2021 | Content Type: Application Notes

This study explores the feasibility of using RADIAN ASAP – a new compact device based on Atmospheric Solids Analysis Probe-Mass Spectrometry (ASAP-MS), for rapid drug screening.

Increased Peak Recovery and Peak Shape of Phosphorylated Compounds Using ACQUITY PREMIER and XBridge...
Library Number: APNT135082027 | Format: PDF | File Size: 475.01 kB | Year: 2021 | Content Type: Application Notes

This application note demonstrates the benefits of PREMIER columns compared to stainless-steel columns.

Improving UPLC-FLR-MS N-glycan Analysis of Disulfide-rich Fusion Proteins Through Optimization of Sa...
Library Number: APNT135082030 | Format: PDF | File Size: 587.5 kB | Year: 2021 | Content Type: Application Notes

Here, we report a new sample preparation method to improve the UPLC-FLR-MS-based N-glycan analysis of fusion proteins exhibiting a high level of disulfide bonds and complex glycoprofiles using a modified protocol for the Waters GlycoWorks RapiFluor-MS N-g...

Analysis of Residual Solvents in Hemp Oil Using Headspace Sampling and Atmospheric Pressure GC-MS/MS
Library Number: APNT135082035 | Format: PDF | File Size: 871.02 kB | Year: 2021 | Content Type: Application Notes

This work will evaluate the use of HS APGC-MS for the quantitative analysis of residual solvents in hemp oil.

Analysis of Vitamin K1 in Serum by UPLC-MS/MS for Clinical Research
Library Number: APNT135082063 | Format: PDF | File Size: 747.87 kB | Year: 2021 | Content Type: Application Notes

A new clinical research method for the analysis of vitamin K1 in serum for has been developed using UPLC-MS/MS with electrospray ionization.

A Green and Comprehensive Approach for Palm Phytonutrients Analysis Using ACQUITY UltraPerformance C...
Library Number: APNT135082067 | Format: PDF | File Size: 522.67 kB | Year: 2021 | Content Type: Application Notes

In this study, we developed a green alternative, more efficient separation method with Waters’ ACQUITY UltraPerformance Convergence Chromatography (UPC2) technology using supercritical CO2 as mobile phase for the analysis of vitamin E, phytosterols, and s...

The Importance of Column Compartment Thermostatting and Preheating for Temperature Sensitive Separat...
Library Number: APNT135082074 | Format: PDF | File Size: 577.26 kB | Year: 2021 | Content Type: Application Notes

This application note demonstrates the effect of differences in column compartment design on resolution and selectivity of a critical pair of temperature sensitive compounds across various LC instrument vendors.

Demonstrating Method Equivalency of the ACQUITY UPLC H-Class PLUS Bio Binary System and an ACQUITY U...
Library Number: APNT135082082 | Format: PDF | File Size: 675.11 kB | Year: 2021 | Content Type: Application Notes

In this work, a RPLC peptide mapping method was used to evaluate a method transferred from an ACQUITY UPLC I-Class PLUS System to an ACQUITY UPLC H-Class PLUS Bio Binary System where retention time and peak area reproducibility were assessed between the t...

Best Practice for the Use of Xevo TQ-S cronos for Residue Analysis in Food – Determination of Triphe...
Library Number: APNT135082079 | Format: PDF | File Size: 1052.06 kB | Year: 2021 | Content Type: Application Notes

In this application note we present a UPLC-MS/MS method using the latest Xevo TQ-S cronos instrument for the accurate and precise quantification of dyes and their metabolites, suitable for checking shrimps for compliance with European regulations.

Evaluation of the Performance of an LC-MS/MS Method for the Determination of Anionic Polar Pesticide...
Library Number: APNT135082087 | Format: PDF | File Size: 462.13 kB | Year: 2021 | Content Type: Application Notes

This application shows the successful evaluation of the performance of this method by interlaboratory study using ACQUITY UPLC systems and Xevo TQ-XS tandem quadrupole mass spectrometers.

Size-Exclusion Chromatography (SEC) of Proteins and Peptides Application Notebook
Library Number: APNT135068236 | Format: PDF | File Size: 34443.85 kB | Year: 2021 | Content Type: Application Notes

In this application notebook, Waters has compiled the following references to help guide through some common challenges and applications associated with SEC of peptides and proteins. The topics covered range from fundamentals of SEC to suggested best prac...