オリゴ核酸のインタクト質量確認と純度測定のためのコンプライアンス対応の自動 LC-MS ワークフロー
This application note describes a streamlined, compliance-ready workflow for oligonucleotide analysis employing the BioAccord System operating under waters_connect HUB data acquisition and processing software.
- A waters_connect workflow is shown to provide good mass accuracy (5–15 ppm) for intact mass confirmation of oligonucleotides of various lengths (10–35 mers), as well as phosphorothioated oligos.
- In addition, the automated processing method that was developed provides purity information for all the oligo sample components that are separated by ion-pair reversed-phase chromatography.
- This automated, compliance-ready LC-MS workflow provides for a robust, easy-to-use solution for fast mass confirmation and purity analysis of oligonucleotides.