Topics in Liquid Chromatography: Part 1. Designing a Reversed-Phase Column for Polar Compound Retention

Library Number:
720001889EN
Part Number:
720001889EN
Author(s):
Patrick D. McDonald, Douglas McCabe, Bonnie A. Alden, Nicole Lawrence, Daniel P. Walsh, Pamela C. Iraneta, Eric Grumbach, Fang Xia and Paula Hong
Source:
Waters Whitepaper
Content Type:
Literature
Content Subtype:
White Papers
Compounds:
Test Mixture: Thiourea; 5-Fluorocytosine; Adenine; Guanosine-5''-monophosphate; Thymine. Uracil, Propranolol, Butylparaben, Naphthalene; Dipropylphthalate; Acenaphthene; Amitriptyline; Norepinephrine; Epinephrine; Dopamine; 3,4-Dihydroxyphenylacetic acid [DOPAC]; Serotonin [5-HT]; 5-Hydroxy-3-indoleacetic acid [5-HIAA]; 4-Hydroxy-3-methoxyphenylacetic acid [HVA]; Cytosine; 5-Fluorouracil; Guanine
Matrix:
Test mixtures
Column:
Atlantis dC18 5 µm Steel 4.6 mm 150 mm Atlantis T3 C18 5 µm Steel 4.6 mm 150 mm Atlantis T3 C18 3 µm Steel 4.6 mm 75 mm ACQUITY UPLC™ HSS T3 1.8 µm Steel 2.1 mm 50 mm

Latest refinements reflect the culmination of more than three decades of proprietary experience in improving retention, selectivity, peak shape, and stability via substrate synthesis, particle morphology manipulation, and surface modification.

Fully aqueous-compatible packings have been made by combining properly tailored silica substrates with a novel, new, T3-bonding process, designed to create bonded phases with enhanced stability in acidic solutions and excellent peak shape. These columns set a new performance standard for polar compound retention and selectivity in HPLC and UPLC™ separations.


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