Simultaneous Measurement of Cyclosporin A and Creatinine Concentrations in Low-Volume Capillary Blood (Finger-Prick) Samples using Liquid Chromatography Electrospray-Ionisation Tandem Mass Spectrometry

Library Number:
WMP241
Author(s):
Cooper, D.P[1];Keevil, B.G[2];Tierney, D.P[2];Machall, A[3];Yonan, N[3];Morris, M.R[1]
Source:
AACC; Orlando; 28th-1st August; 2002
Content Type:
Posters
Content Subtype:
AACC
--Routine monitoring for the immunosuppressive drug cyclosporin A (CsA) is required to maintain an effective, sub-toxic drug concentration. --Current practice is to measure trough concentrations of CsA but C2 measurements (2 hours (plus/minus) 10 minutes post-does) are advocated as a more informative indicator of drug exposure. --In a busy clinic, accurate timing for C2 sampling could present considerable difficulties. --C2 monitoring and pharmacokinetic profiling could be facilitated by using capillary (finger-prick) blood sampling but current immunoassays do not have sufficient sensitivity and additional test for kidney function would be be required. --We have therefore developed and validated a liquid chromatography- tandme mass spectrometry (LC-MS/MS) method for the simultaneous, routine analysis of CsA and creatinine in low-volume (finger-prick) whole blood samples.

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