Rapid Identification of Bacteria by IntactCell Matrix Assisted Laser DesorptionIonisation Time of Flight Mass Spectrometry

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Edwards-Jones, V[1];Morgan, M.M[1];Bright, J.J[1];Dare, D.J[2];Keys, D.J[2];Shah, H.N[2]
IBMS; Birmingham, UK; 25th-27th September; 2001
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Characterisation of bacteria using intact cell matrix assisted laser desorption ionisation time of flight mass spectrometry (ICMS) is a newly described rapid identification method. ICMS uses the surface ions generated from the soft-ionisation of the intact bacterial cell to generate apparent reproducible fingerprint spectra within a few minutes(1). There is sufficient data within the spectrum to identify the bacterial species and further studies have shown that for some bacterial species, the generated fingerprints are sufficiently discriminatroy to allow simultaneous sub-typing (2,3). To be successful, the bacterial mass spectral fingerprint must be reliably matched to a spectrum of the same species in a database. A large database of quality controlled spectral fingerprints of bacteria of know provenance (NCTC strains) was created and challenged with a data from a selection of the same bacterial strains generated at a different point in time, on a different instrument. In addition, the database was challenged with several clinical isolates. This study describes the preliminary findings.

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