Solid-Phase Extraction and Cleanup Procedures for the LC/MS Determination of Acrylamide in Fried Potato Products.

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Michael S. Young;Kevin M. Jenkins, Jim Krol [Waters]
HPLC 2003, AOAC 2003
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Content Subtype:
Oasis HLB 200 mg 6 cc Vac Cartridge Oasis MCX 60 mg 3 cc Vac Cartridge
Atlantis dC18 5 µm Steel 2.1 mm 150 mm
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Recently, it has been discovered that many commercially available fried potato products contain variable levels of acrylamide. The acrylamide is presumed to be produced by the reaction of certain natural constituents of the food when processed at high temperatures. Because acrylamide is considered to be a possible carcinogen, there is a need for rapid, reproducable and rugged analytical methods for determination of acrylamide in food. Acrylamide is a highly polar and highly water soluble substance. Because of its highly polar nature, direct GC analysis of this substance is difficult although sensitive analysis of this substance has been accomplished using GC or GC-MS after derivatization with bromine. Because the GC analysis is rather cumbersome and time-consuming, LC analysis for acrylamide may be preferable. Although LC-UV analysis of this substance has been accomplished in some matrices, UV detection is not sufficiently sensitive for routine acrylamide analysis in foodstuffs. Moreover, acrylamide shows only limited retention behavior by reversed-phase LC (RPLC), even with highly aqueous mobile phases. This presentation will discuss the determination of acrylamide in potato chips and other foodstuffs using SPE for sample enrichment and cleanup followed by LC-MS analysis. The procedure involves initial extraction of the foodstuff with aqueous 2 M NaCl. An aliquot of the initial extract solution was loaded onto an Oasis HLB (reversed-phase) cartridge. After an aqueous wash step, the cartridge was eluted with acidified methanol and was passed through a mixed mode cation-exchange cartridge (Oasis MCX). After evaporation of the eluate, the residue was reconstituted in 250µL of water for RPLC on an Atlantis dC18 analytical column (4.6 x 150 mm, 5 ìm particle size).

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