A Study of Band Broadening Effects in HPLC
The van Deemter equation has been used extensively to describe
the relative magnitude and sources of dispersion leading to band
broadening in HPLC. The magnitudes of the reduced terms in the van
Deemter equation will be compared across bonding chemistry, particle
size (2.5µm to 5µm), and particle composition (silica versus hybrid).
The data will be reviewed in light of papers published over the past 5
years concerning the major sources of dispersion: static zone versus
mobile zone. The acquisition of data that accurately describes band
broadening in the chromatographic column requires an in depth
understanding of the limitations of all components of the
chromatographic system. The effects of system dispersion, injection
delays, digital conversion of data, and choice of column configuration
on the data will be described.