A Review of the Chromatographic Performance of the State of the Art Reversed-Phase Silica Packings

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Pamela Iraneta;Bonnie Alden;Cheryl Boissel;James Cook;John O’Gara;Dan Walsh;Susan Serpa;Tom Walter [Waters]
HPLC 2003
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Content Subtype:
maleic acid; quinine; amitriptyline; perphenizine; meclizine; phenol; desipramine; nortriptyline; salicylic acid; nortriptyline; acenaphthene; dipropylphthalate; naphthalene; butylparaben; propranolol
It has been over 15 years since the introduction of high purity silica-based reversed-phase packings. These high purity silica packings were introduced to address the problem of poor peak shape for basic compounds. Improvement in peak shape for basic compounds has continued to be an area of interest and development. Tailing or asymmetry factors alone inadequately describe differences in performance due to residual silanols in these improved packings. In this study the chromatographic performance of the current state of the art reversed-phase silica packings are compared using selectivity, efficiency and asymmetry. The chromatographic performance will be evaluated for basic, acidic and neutral compounds using simple mobile phases. Peak shape for basic compounds will be investigated as a function of flow rate. Accelerated high and low pH stability data will be presented.

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