Intelligent Speed: Increasing Sample Throughput and Reducing Laboratory Costs

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Diane M. Diehl, Kim Van Tran, Eric S. Grumbach, Uwe Neue, Jeff Mazzeo [Waters]
AAPS, Salt Lake City Utah, October 26-30
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Caffeine; Aniline; N-Methylaniline; 2-Ethylaniline; 4-Nitroanisole; N-N-Dimethylaniline; Cinoxacin; Oxolinic Acid; Nalidixic Acid
Today’s pharmaceutical chemists are being asked to increase productivity and reduce their laboratory costs. In the area of high-performance liquid chromatography (HPLC), one way of achieving these requests is to reduce the overall runtime of a separation. One way to go faster is to use inexpensive cartridge systems or guard columns in lengths under 30 mm. However, this design is not as rugged as a column and these cartridges are not individually tested. This is why cartridge systems often do not give reproducible results, which can be detrimental. We have developed new Intelligent Speed (IS™) columns in 20 mm lengths packed with a variety of stationary phases that offer excellent peak shapes, reproducibility and fast separations for high sample throughput We developed a gradient separation for 6 analytes on a 4.6 x 150 mm column with a cycle time of 25 minutes. We then scaled the gradient and ran the separation on a 4.6 x 20 mm IS™ and a 2.1 x 20 mm IS™ column with a total cycle time of 3 minutes. We also performed calculations on the increase in sample throughput as well as the reduction in costs. To show the usefulness of narrowbore chromatography in LC/MS applications, we developed several separations on 2.1 x 20 mm IS™ columns with direct flow into the MS. In the scale down separation, we reduced our cycle time by a factor of 8. This results in an 8-fold increase in sample throughput. We also determined that we can save thousands of dollars in solvents by using shorter columns. Using the narrowbore columns, we can reduce LC/MS runtimes, as well as flow directly into the MS, reducing the need for flow splitting. Fast separations that increase sample throughput and reduce laboratory costs can be achieved using the new 20 mm length Intelligent Speed (IS™) columns. Separations originally run on long columns can be scaled down and run on the IS™ columns with runtimes of under 5 min. New methods can also be developed on the columns, saving time in method development. Additionally, these columns can be used to generate fast LC/MS applications with direct flow into the MS.

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