Ultra Performance Liquid Chromatography (UPLC) Coupled with OA-TOF-MS for Metabolite Identification

Library Number:
Part Number:
Michael A. McCullagh, Lisa Southern, Hilary Major, Jose Castro-Perez (Waters);John Lindon, Jeremy Nicholson, Catherine Duckett (Imperial College of Science, Technology and Medicine);I. Wilson (AstraZeneca)
Montreux 2004
Content Type:
Content Subtype:
Related Products:
A comparison of the response and chromatographic separation obtained using conventional HPLC and UPLC™ coupled with oa-TOF has been performed. Utilising UPLC technology and columns packed with particles <2 µm, high efficiency separations can be achieved at high flow rates, allowing superior resolution and sensitivity to be obtained in a shorter analysis time. UPLC generates chromatographic peaks that are typically only several seconds in width, which places demands upon the detection technique to acquire data at an appropriate rate. The fast duty cycle of oa-TOF technology allows 20 spectra/second to be acquired generating sufficient sampling points across a peak to produce a representative response. An application area has been selected, where previous studies have been performed to illustrate the reduced retention times, improved chromatographic resolution and increased MS response. This poster was shown at: Montreux LC/MS Symposium on LC/MS, SFC/MS, CE/MS and MS/MS, Montreux, Switzerland, November 10-12, 2004.

Title Format File Size
720001004en PDF 255.67kB