The development of new packing materials requires the use of standardized chromatographic tests in order to assess the performance of the new packings. Our standard tests include: van Deemter experiments; selectivity and peak shape at low and intermediate pH; and stability at high and low pH. Development of a new high-pH stability testusing NaOH at pH 12.3was required due to the dramatically improved high-pH stability conferred by the bridged ethyl hybrid (BEH) particle and improved bonding technology.
Data from our evaluation tests will be presented for these new BEH stationary phases. Unlike commonly used bonding approaches, our improved bonding technology confers excellent stability at low and high pH. In methods development, pH has long been recognized as a major parameter influencing selectivity. The presented data supports the use of several BEH stationary phases across an expanded pH range.