Separation of Polar Analytes on Preparative RPLC Columns

Library Number:
Part Number:
Fang Xia*, Jie Y. Cavanaugh, Damian Morrison, Martin Gilar, Uwe D. Neue, Jeffrey R. Mazzeo, Diane M. Diehl [Waters]
Pittcon 2004
Content Type:
Content Subtype:
sulfanilamide, sulfathiazole , sulfamethazine, sulfamethoxazole, sulfonamides
Atlantis dC18 5 µm Steel 4.6 mm 100 mm Atlantis dC18 5 µm Steel 19 mm 100 mm
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Very polar compounds are difficult to retain and separate using traditional reversed-phase columns (e.g. C18 stationary phase) due to the inability to effectively retain such compounds in a fully aqueous mobile phase. The design and development of a new silica-based reversed-phase (RP) packing material designed for retaining and separating the highly polar compounds was successfully achieved on a series of columns with analytical dimensions. In this study, we will present our applications of separating polar compounds on this stationary phase in preparative column dimensions. This data will include the separation of low molecular weight compounds used in the pharmaceutical industry, the separation of hydrophilic peptides. In addition, to demonstrate the universal applicability of this stationary phase material, separations of compound mixes consisting of polar and non-polar analytes will also be shown. Several factors that affect column loading and resolutions are also studied. The new type of preparative RPLC column imparts high mass loading, high peak capacity and excellent peak shape with MS compatible mobile phase.

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