UPLC with MS (TOF): New Separations Technology for High Throughput Metabonomics

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Waters Corporation: Jennifer Granger, Robert Plumb, Jose Castro-Perez. AstraZeneca: Rebecca Williams, Eva M. Lenz, Ian D. Wilson.
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Metabonomics is a rapidly expanding area of scientific research, which has the potential to improve the drug discovery process by providing an insight into the mechanisms of toxicity and efficacy. The approach relies on comparing the endogenous metabolic profiles of mammalian systems following exposure to an external stimuli, e.g. a candidate pharmaceutical or environmental stress, using chemometric data analysis strategies. Much recent interest has been focused on LC/MS analysis methods as an alternative to H-NMR. An additional resource for LC/MS based metabonomics has been found in Ultra Performance Liquid Chromatography (UPLC). UPLC offers several advantages, namely improved chromatographic resolution, increased sensitivity and reduced analysis times. This is achieved through the use of small particle sizes (< 2um) and pumping-injection systems capable of operating under such exacting pressure regimes (up to 15,000 psi). When coupled with time of flight mass spectrometry, UPLC presents a means to achieve high sample throughput with reduced spectral overlap, increased sensitivity and exact mass detection capabilities.

This poster was presented at ISSX Drug Metabolism in Vancouver on Sept. 1, 2004.

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