Enhancing Mass Spectral Analysis of Trace Peptides with Optimized Chromatography

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Thomas E. Wheat, Jo-Ann M. Jablonski, Beth L. Gillece-Castro, and Diane M. Diehl, Waters
Mass Spec 2007 Sept. 5-7 Montreal Canada
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Other Symposium
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Peptide mapping with reversed phase HPLC is an established technology for the analysis of protein structure. Coupling the chromatographic separation to mass spectral detection synergistically increases the information available. In characterizing variant structures in a protein sample, complications can arise. Low abundance peptides, representing trace modifications, may be overlooked or lost in the more intense signal representing the native structure. We have optimized peptide separations to ensure the highest quality spectral data for characterizing trace modifications of a protein. An essential element of this optimization is UPLC peptide mapping for higher resolution than is possible with HPLC. This poster was shown at Mass Spec 2007: Fourth Symposium on the Practical Applications of Mass Spectrometry in the Biotechnology and Pharmaceutical Industries.

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