Practical Considerations in the Application of UPLC to LC/MS Peptide Mapping
Peptide mapping with reversed phase HPLC is an established
technology for the analysis of protein structure. Coupling the
chromatographic separation to mass spectral detection synergistically
increases the information available. In characterizing variant
structures in a protein sample, complications can arise. Low
abundance peptides, representing trace modifications, may be overlooked
or lost in the more intense signal representing the native structure.
We have optimized peptide separations to ensure the highest quality
spectral data for characterizing trace modifications of a protein. An
essential element of this optimization is UPLC® peptide mapping for
higher resolution than is possible with HPLC.