Fractionation of Neutral and Sialylated N-Linked Glycans Using Micro Scale HILIC SPE for MALDI-TOF MS Analysis

Library Number:
720002352EN
Part Number:
720002352EN
Author(s):
Ying Qing Yu, Jennifer Fournier, Martin Gilar and John C. Gebler, Waters
Source:
Mass Spec 2007 Sept. 5-7 Montreal Canada
Content Type:
Posters
Content Subtype:
Other Symposium
A micro scale hydrophilic interaction chromatography (HILIC) SPE method has been developed to fractionate and enrich N-linked glycans released from glycoproteins.  Low and high pH stepped fractionations enable to isolate the sialylated glycans, therefore, simplifying the identification and structural characterization of these glycans using MALDI MS analysis. This poster was shown at Mass Spec 2007: Fourth Symposium on the Practical Applications of Mass Spectrometry in the Biotechnology and Pharmaceutical Industries.

Title Format File Size
720002352en PDF 640.96kB