Evaluation of Reversed-Phase Columns for Protein Separation Using a Single LC/MS - LC/UV Methodology
HPLC separations on reversed phase columns are an important
part of characterizing complex protein samples from basic research and
pharmaceuticals. Recent advances in mass spectrometry have enabled
rapid characterization of protein and peptide samples separated by
HPLC, greatly facilitating the identification of target proteins and
contaminants. Thus, there is a need for good separation techniques
using MS compatible methodology for the separation of proteins by HPLC.
We present here the evaluation of peak capacity and recovery in protein
separations along with LC/MS. Our findings resulted in the
determination that both Delta-Pak and Symmetry300 columns are preferred
for performing LC/MS protein separations.