Detecting Carbamylation of Intact Proteins and Peptides by LC/MS (/MS)
Characterization of pharmaceutical proteins is an important
analytical challenge that must be met in pharmaceutical and
biotechnology companies today. Small chemical variations in proteins
can be the difference between an active and an inactive therapeutic.
Reversed-phase chromatography coupled with mass spectrometry has
rapidly become the technique of choice for the determination of post
translation modifications. We have developed a simple and rapid LC/MS
method for identifying the presence and degree of carbamylation of
intact proteins or peptides in a mixed sample using either single
quadrupole or Q-TOF type instrumentation.