Ion-Pair Reversed-Phase High Performance Liquid Chromatography of Dye-Labeled Oligonucleotides.

Library Number:
WA20711
Part Number:
WA20711
Author(s):
Kenneth J. Fountain;Martin Gilar;Yeva Budman;John C. Gebler
Source:
FAll ACS 2002; 224th ACS National Meeting; Boston August 18-22
Content Type:
Posters
Content Subtype:
ACS
Compounds:
Synthetic Oligonucleotides; 6-Carboxyfluorescein; 6FAM; Hexachlorofluorescein; HEX; Tetrachlorofluorescein; TET; Carboxytetramethylrhodamine; TAMRA; Indodicarboxycyanine; CY3
Column:
XTerra MS C18 2.5 µm Steel 4.6 mm 50 mm
Related Products:
Singly- and dually-labeled synthetic oligonucleotides were purified by ion-pair reversed-phase high performance liquid chromatography using a 50 × 4.6-mm column packed with porous, 2.5 um C18 sorbent. We studied the mechanism of dye-labeled oligonucleotide retention in order to improve the quality of purification. We purified oligonucleotides labeled with 6-carboxyfluorescein (6FAM), hexachlorofluorescein (HEX), tetrachlorofluorescein (TET), indodicarboxycyanine (Cy™3) and carboxytetramethylrhodamine (TAMRA) dyes, as well as dually labeled TaqMan probes. Purification of a 0.1 umole crude oligonucleotide synthesis in a single injection was demonstrated.

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