Improved LC/MS throughput with on-line sample cleanup, peak focusing and composition and flow gradients

Library Number:
Part Number:
Li, Jeanne B.;Block, Eric H.;Balogh, Michael P.
ASMS 2000, Long Beach, CA; June 11-15
Content Type:
Content Subtype:
Diphenhyramine; Oxybertymin; Terfenadine; Acetonitrile
Calf serum; Hanks solution (salt solution)
Oasis® HLB 30 µm Extraction Column - steel 2.1 mm 20 mm XTerra MS C18 3.5 µm Steel 2.1 mm 10 mm XTerra MS C18 3.5 µm Steel 2.1 mm 30 mm
More LC/MS samples need to be analyzed per day. Many samples contain complex matrices. Removing the matrix can 1) reduce ion suppression from salts, 2) keep the MS sample cone clean longer, 3) reduce background ions. This can lead to greater detection sensitivity. For some basic analytes a gradient separation at high pH is better for peak shape and resolution than acidic pH. The availability of solid phase extraction and analytical columns that are stable from pH 1 to 12 and can withstand rapid pressure changes (0 to 4000 psi) increases possibilities for methods development. With column switching valves and flow programming, it is possible to perform on-line sample cleanup and fast gradient separations with cycle times of 3 to 5 minutes. Rapid equilibration and off-line column regeneration can decrease cycle time.

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