Fast Analysis of Aldehydes and Ketones using UPLC

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Mark Benvenuti and Joe Romano
NEMC 2006, Aug. 28-31, Arlington, Va.
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Other Symposium
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Aldehydes and ketones are products of combustion that permeate the environment. A number of these compounds are known carcinogens and, as a result several USEPA and state methods have been developed for their analysis. These methods, US EPA TO5 ( air ), 554 ( drinking water ), 8315A, Options 1 and 2, ( waste water,soil and air ) along with California Method 1004 ( carbonyl compounds as alcohol oxidants in auto exhaust ) describe the derivatization of these compounds using Dinitrophenylhydrazine ( DNPH ) followed by UPLC separation and UV detection at 360 nm; target analytes vary slightly by method. Present HPLC methods describe run times of over 40 minutes and multiple column setups to achieve acceptable analyte resolution. This poster demonstrates that with UPLC analysis time can be reduced by as much as 75%. In addition, excellent resolution is achieved for acetone, acrolein and propanal in Method 8315A, Option 2 and methacrolein and MEK in California method 1004. This poster was presented at the National Environmental Monitoring Conference, Aug. 38-31, 2006, Arlington, VA.

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