A Label-Free Approach to the Quantitation of Proteins from Methicillin Sensitive (MSSA) and Methicillin Resistant (MRSA) Staphylococcus Aureus Strains by LC/MS

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Thérèse McKenna, Christopher Hughes Alistair Wallace, James Langridge;(Waters) V. Encheva, H.N. Shah (Health Protection Agency, Centre for Infections, Molecular Identification Services Unit, London UK)
IMSC 2006, Aug. 27-Sept. 1, Prague
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We employed a ‘label-free’ mass spectrometrybased approach to analyze the proteomic profiles from a total tryptic digest of Staphylococcus aureus (MRSA) and (MSSA) for the detection of potential markers for strain differentiation. The overlap of proteins identified between the strains was investigated. A number of putative biomarkers were identified and quantified. The confidence limits of these potential markers for clinical diagnostics will be established in larger studies.

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